Elementary Analysis And Gene Mapping Of Anthocyanidins Of Yu Nan Hei Xiang Nuo

Black rice, which contains rich anthocyanidin, special nutriment, vitamin and health care effects, had been planted and edible for the long history in China. Black rice shows specific pigment because of depositing massive anthocyanidin on the perciarp of caryopsis. With contents of anthocyanidin increasing, caryopsis shows different pigment. In addition to, it is reported that content of anthocyanidin directly affected content of trace element. Thus, it is very important to analyze the changes of content of anthocyanidin and the genetic mechanism in view of the special functional feature of black rice for the people.In this paper, the changes for content of anthocyanidin ingredient affected by the other factors on different develomental phase from Yunan heixiangnuo was analyzed. On the other hand, we analyzed the genetic law by using the F₁ and F₂ progeny populations between black rice (Yunan heixiangnno) and common rice ( Shuihui527). Furthermore, the pigment gene of black rice was mapped on the chromosome by microsatellite mark. The results were as follows:1. According to correlation analysis of anthocyanidin, proanthocyanidin and flavone, which were the key factor to pigment, the result showed they were significant difference at the level 5% between anthocyanidin and proanthocyanidin, and they were significant difference at the level 1% between anthocyanidin and flavone.2. Anthocyanidin, proanthocyanidin and flavone, which were analyzed in vegetable growth, the change for content of them showed S curve in sheath. Meantime, the maximum content of anthocyanidin and flavone were on tillering stage, 27.1041U/g and 6.9826g/kg, respectively. But the highest content of proanthocyanidins was on culm enlonged stage, 149.0104g/kg.3. Anthocyanidin, proanthocyanidin and flavone, analyzed in reproductive development, they showed straight raise in caryopsis. And the maximum content of anthocyanidins, proanthocyanidins and flavone were on maturity stage, 52.6193U/g, 472.3193g/kg and 31.1150g/kg, respectively.4. Genetic analysis and gene mapping were held on pigment of Yunan heixiangnuo. Pericarp of F₁ showed black by orthogonal cross between black rice and white rice, but pericarp of F₁ showed achromatism through back-cross. Thus, pigment gene of pericarp was determined by female parent. On the other hand, the segregation ration of F₂ population accorded with 3:1 whether it is orthogonal cross or back-cross, indicated that the black pigment trait was controlled by a pair of dominant gene.5. The F₂ population derived from the cross"Yunan heixiangnuo×Shuhui527"was selected as the mapping population. The whole genomic DNA for bi-parents were amplified with 600 pairs of SSR primes distributed on 12 chromosomes in rice. Polymorphic primers were used to analyze F₂ recessive population. The software of MAPMAKER 3.0 was used to construct linkage map, the purple pigment gene was located between RM19569( the genetic distance was 1.2cM) and RM19565( the genetic distance was 0.7cM) on the chromosome 6.