Genetic Mapping And Position Effect Of Rice T-DNA Integration Genetic Analysis And Gene Mapping Of A Rice Multi-pistil Mutant

Part Rice (Oryza Sativa L.) is one of the most important food crops in the world. The transformation procedure mediated by Agrobacterium has been successfully applied to rice in recent years. In the previous research we have transfered the rice bacterial blight resistance gene Xa21 into eight rice varieties of China by using Agrobacteriunrmediated transformation. In this study, T-DNA flanking sequences of the Xa21 transgenes in transgenic rice lines were obtained by using thermal asymmetric interlaced PCR (TAIL-PCR). The flanking sequences, which are actual rice DNA, were identified and located on molecular linkage map developed from a ZYQ8/JX17 double haploid (DH) population. A total of 22 T-DNA flanking rice sequences were isolated. Nineteen of them displayed RFLPs between the two parents, ZYQ8 and JX17, and were mapped on the rice chromosomes, 3, 4, 7, 9, 10, 11 and 12, respectively. The genetic mapping of /a