The Preliminary Study On The Influence Factors Of Extraction Methods And Content Of Flavones And Polysaccharide In Moringa Oleifera

Aim to stabilize and improve the products by effective content of Moringa oleifera, which is from the ecological park Yingdong of Shaoguan University in Guangdong planting Moringa oleifera of two years. We found that the ethanol circumfluence for flavones determining and Phenol-sulferic acid method for Polysaccharide were the optimal extraction technology. Addition to this, the relationship of the Leaves, organ, producing area, Harvest time, and levels under the management, position with the effective component content would be discussed. The results showed that:The optimal extraction technology of total flavones from the leaves of Moringa oleifera was: 70% ethanol, 20 times volume, 80℃, 90 minutes and extract three times. The yield of total flavones was 6.59% under this optimal extraction condition.The optimal extraction technology of polysaccharides from the leaves of Moringa oleifera was:15 times volume waters, 90℃, 120 minutes and extract three times. The yield of polysaccharide content was 24.76% under this optimal extraction condition.The total flavones and polysaccharide content of Moringa oleifera were the highest on the stronger leaves, which 45 days older. The total flavone was 6.11% and polysaccharide was 21.97 % on these leaves. Otherwise, these contents were less on the younger leaves and older leaves.Total flavones content of Moringa oleifera in different organs were showed that: the flower-petiole is most, root is least, and the variety bound were 0.53%～4.47%. And the polysaccharide content on the roots were most, the petiole were least, and the variety bound were 8.16%～33.61%.The effective components on the different Producing area were: the total flavones were the highest in the materials from the west suburb-racing factory. In which the leaves were 6.78%, petioles were 3.43% and stems were 2.66%. But the polysaccharide in the Xinfeng factory was the highest levels. The leaves were 27.14%, petioles were 16.19 %, and stems were 12.28%. The effective components were the least which from the Yingdong ecological park of Shaoguan University.There was a difference of the effective components within different harvesting period in our experiment. It's showed that the effective component content was the highest harvested in November. The total flavone of leaves were5.69%, the petiole were 3.13 %, and stems were2.01 %. The polysaccharides of leaves were 28.85%, the petioles were 9.71 %, and the stems were 12.24 %.The effective components of different levels under the management levels were showed that: the leaves under middle management levels were the most. Otherwise, the sequence of the effective component content in the petioles and stems under different management levels were: Low>middle> high.There was no significant difference of the effective component content in the different orientations.