| Strawberry is perennate herbacecous plant which belongs to rosaceous Branch ,Fragarial herbs and is called small berries in horticulture, The strawberry fruit has bright color , moderate sour and sweet flavor, rich aromatic and nutrient. It's one of the world's seven famous fruits. China's strawberry production has developed rapidly in recent years, the cultivation area has been expanded. Jinhua has the subtropical monsoon climate, abundant energy, abundant rainfall, four distinct seasons, obvious dry and wet season, which are good for strawberry cultivation. In recent years strawberry cultivation developed rapidly in Jinhua, but the strawberry tend to be infected by the virus because it's an asexual reproduction plant. According to statistics, strawberry degradation reduction is 30% -80%because of infection. Using chemical drugs to cure the disease have little effect, the virus-free seeding are generally used to control the spread of the virus in production. One of important ways to get virus-free strawberry plants is to combine the heat treatment with the stem tip culture. This article outlining the strawberry production of home and abroad ,inside and outside province and jinhua on the base of much reference, while making a series of studies on the detoxification breeding technology of strawberry by combining the heat treatment with the stem tip culture. The main conclusions are as follows:1. The strawberry stem tips were placed in a water bath of 35℃, 40℃, 42℃. 45℃, 50℃for 4 h, observed the survival rate and identified the detoxification results ,determined the most appropriate heat treatment temperature is 40℃.2. BA2.0, NAA0.2 (mg / L) is more appropriate choice during virus-free strawberry stem tip-induced bud differentiation in experiment. In different stages of subculture, the concentration of hormones is gradually reduced with the subculture increased. During the experiment, the BA concentration is 2.0mg / L, NAA concentration is 0. 2mg / L before subculture, with the increasing subculture. BA concentration gradually reduced to 0.5 mg / L. NAA concentration dropped to 0.05 mg / L, we get a good multiplier effect. And taking 1 / 2 MS + IBA0.5 + IAA0.2 (mg / L)as rooting medium gets the best effect. 3. Carrying out the low-cost training experiment of a Virus-free seeding, focus on the rooting stage .The test showed that using simple medium which has the liquid but solid, sugar but sucrose, cold water but distilled water. This medium has no apparent impact on rooting and survival rate for transplanting, and the costs are greatly reduced, therefore, the simple medium can be widely applied in production.4. Make a comparative test of transplanting the strawberry seeding to different base material, we mach the most common material, peat soil, mud, sand to form different base material, filtering out the best base material which has low cost and high survival rate, and the seeding grows well with it—peat soil: sand =1:1.5. Carrying out the field experiment with virus-free seedlings, the virus-free seeding can recover nature, grow strongly, has no virus disease in growing stage, the virus -free seeding has more positive advantages than the contraposition in weight, the root number, the leaves number, the leaves area and stem diameter ,and the production of was increased by 23 percent at least.
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