Study On Tissue Culture And Detoxification Of Sugarcane Badila

The study was based on the Badila,the dominant species in Guangdong province,and the young leaves and stem apical meristems as explants.In the first stage,induction of the callus differentiation into seedlings,the callus induction,differentiation,and proliferation of clustered shoots were used to investigate the suitable medium formulation and hormone ratio during the tissue culture of cane.In the second stage,different conditions of heat treatment were applied to the axillary buds and tissue culture seedlings,comparison of the growth status of the axillary buds and tissue culture,the survival status of the stem tip,the induction of stem tip callus,and the growth status of shoot tips.The effects of different heat treatment on the stem tip detoxification of the fruit cane and the seedlings were compared,in order to provide some theoretical basis for the improvement of sugarcane varieties.The results of the study are as follows: 1 sugarcane tissue culture induced test results:(1)In the callus induction test of young leaves of the cane,the MS+2,4-D1.5~3.0mg/ L medium was the best,and the callus recovery rate was the highest,87.5%.The color of the callus is pale yellow,loose particles and best quality.(2)In the proliferative test of cane callus,the appropriate combination of 2,4-D and NAA can effectively promote callus growth.The best medium was MS+2,4-D2.0mg/L+N AA0.01~0.05mg/L.The callus was white or light yellow,with loose particles and uniform texture.The highest breed coefficient was 4.88.(3)In the callus differentiation test of sugarcane,the combination of 6-BA and NAA hormone induced the callus differentiation rate was significantly higher than that of 6-BA alone,and the growth condition of the clustered shoots was good.When adding MS + 6-BA1.0 mg/L + NAA0.1 mg/L ratio of hormone,sugarcane callus differentiation rate is highest,at95%.The clustered shoots obtained have good growth and erectness.The leaves are dark green,and the sprouts are high and strong.The height of the shoots is greater than 2 cm.(4)In the proliferation experiment of sugarcane sprout seedlings,when 6-BA concentration of 1.0~2.0 mg/L was added to the MS medium alone,The proliferation coefficient of the seedlings was the highest,reaching 4.95,and the seedlings were tall and strong,with many tillers and no vitrification.2 Results of detoxification test of sugarcane tissue culture:(1)In different heat treatment conditions,the germination rate of axillary buds was highest at 93.33% in a water bath at a temperature of 48°C for 10 minutes.The germinated axillary buds were thick,and the leaves were dark green with 2~3.The young leaves were buds with an average height of 10 cm.(2)In the test of growth condition of seedling tissue culture plants under different heat treatment conditions,the tissue culture seedlings of fruit cane were cultured at a temperature of 30°C for 25 days,and the survival rate was the highest at 95%.The tissue culture seedlings were erect and stout,and their growth was good.It is appropriate to do stem point meridional tissue flaking material.(3)The size of the shoot cane tip directly determines the degree of difficulty in the callus induction at the shoot tip.Different stem tip size under the same heat treatment conditions,stripping 1.0mm stem tip size,the highest callus rate,up to 83.33%.(4)The optimum medium for the callus induction of stem tip was: MS+2,4-D1.5mg/L,and the stem tip callus was 96.67%.(5)The best medium for seedling of sugarcane tissue culture was 6-BA1.0 mg/L+ NAA0.1mg /L,and the combination of the stem tip was directly formed into the seedling,and the leaf color was dark green,thick and strong,and the differentiation rate was 96.67%.(6)All the heat treatment conditions in this study,the strip size 0.3 mm stem tip,stem tip callus induction and differentiation of stem stem tip seedlings,cultivate cultured from detection by PCR technology,results show that the SRMV with SCMV two virus-free rate reached 100%.(7)Adventitious root induction of shoot clusters in sugarcane,the best medium formulation is MS+NAA2.0mg/l+IBA0.5mg/l+PP3330.5mg/l+0.1% AC,the best induction effect of adventitious roots,the average root number reached 6.15 root/strains,roots are numerous and stout,shoot tip cluster buds growing well?...