| Contents of this paper are as follows: (1) Using flax (Linum usitatissimum L.) (shuangya-5) as explants, dedifferentiation of the seeds and differentiation of its callus through tissue culture technology, as well as proper conditions for a complete developmental process of flax somatic embryogenesis were studied. It would have laid a good platform for the somatic cell hybrid breeding of flax as a parent ; (2) Flax salt-induced mutation cell lines had been inducted by high salt method, and it was proved with physiological and biochemical indicators.Results are summarized as follows:1. Embryonic callus of flax could be gained on the MS medium with 0. 5 mg·L-1 NAA and 0. 5 mg·L-1 KT, and grew in a better state:hazel, dry on surface and loose.2. The callus were induced to yield somatic embryos on the differentiational MS medium with 1.5 mg·L-1KT and 1.0mg·L-1 2.4-D, development of embriod underwent globular embryo, heart-shape embryo, torpedo-shape embryo and cotyledon– shape embryo .But some secondary abnormal unipolar embryos were found in the process.3. Salt-tolerant flax callus could suffer 250mmol·L-1LNaCl was gained. By comparing with moisture content, relative growth rate, soluble sugar content, soluble protein content, proline content, MDA content, POD, CAT and SOD activity, EST isozyme, POD and SOD isozymes , many obvious differences between salt-tolerant callus and ordinary callus were observed, and salt-tolerant mutant cell line of flax was gained. |
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