| Maize is important crop, and it is sensitive to salinity. In our country,amount of plough are salines. To improve the adaptive of maize to salinity, breeding new NaCl-tolerant varieties of Maize is urgent. An project on selecting salt-resistant Maize was put on in our lab. The calli were induced from the immature embroys of maize. After the calli were propagated,they were subgenerated in the N6 medium containing NaCl for three times to select NaCl-tolerant variants. The content of NaCl was increased from 1.0%-1.5%-2.0% one by one, then the NaCl-tolerant variants were transferred to NaCl-free medium for three times subgeneration. The relative growth rate of calli ,the content of proline , the ratio of dry material and waterish weight were analysised.The results showed that when increasing NaCl concentration ,the relative growth rate decreased, the proportion of dry weight to fresh weight, the content of pro rised. While suffering the same salt stress, the varietants accaculate more pro and grew more quickly than the control. It proved the calli were NaCl-tolerant indeed. RO plants were regenerated from NaCl-tolerant callus variants and RI seeds were obtained. The relative electronic conductivity of leaves of the RO variant was lower than the control, but solution sugar content was higher than the control under 1.0%NaCl stress. The germinating rate of R1 generation seeds, the variants were higher than the control, especially the germinating rate of the variant was 20% under 1.6% NaCl pressure, but that of control was zero. In Comparing of the control under the same NaCl stress, the variants seedling had more roots, their seedling were higher. The pro content of the calli and RO plantlet were ten times higher than that of R1 plantlet. However the pro content of the Variants were always higher than that of control. All of the indexes showed that the calli variants and their regenerated plants aresalt-tolerant, and the character of salt-tolerant was inheritable. |
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