Study On Parameters Optimization In Liquid Culture And Inoculation Device For Edible Fungi

At present, most of the edible fungi factories in China are still using solid spawns in cultivation, but they have not been industrialized. In order to apply liquid spawns into edible fungi factories, the nutrition demands and environment conditions of Coprinus comatus (Mull.:Fr.) Gray were studied and an integrative device of edible fungi liquid spawns cultivation and inoculation was designed in this thesis.The main conclusions are as follows:(1) It turned out that the mushroom of RJ had the smallest mycelium and the most the highest mycelial yield. So the mushroom of RJ was the best among five different varieties of baiji, beiji, xingye, fangji, RJ for liquid culture.The mycelial yield and deoxidize sugar were measured during the culture process. It showed that the best time for liquid culture of RJ was five days.(2) The best medium of RJ was known from the single-factor and orthogonal experiments, results showed that the best medium is as follows glucose 2%, corn flour 2.5%, yeast extract 0.5%, bran 3%, KH₂PO₄ 0.25%, MgSO₄0.1%, VB₁ 0.05mg/L and bean oil 0.5%.(3) The suitable temperature, pH, DO were studied through the single-factor and orthogonal experiments, the results showed that the most suitable condition were the temperature of 24°C,pH 6.5, and with 400ml medium in 500ml flask. When culturing Liquid spawns in ten liters, the lag phase was shortened by twelve hours, the best culture time was shortened by thirty-six hours and the mycelial yield was increased by 41.28%, compared with culturing them in flasks.(4) An integrative device of edible fungi liquid spawns cultivation and inoculation was designed, which realized the automation of inoculation, the adjustment of inoculum and the exchange of the mouth of inoculation.