The Cloning And Expression Analysis Of CO/FT Homologous Genes In Phyllostachy Violascens

In this study, homologous genes of CO/FT in Phyllostachy violascens were cloned with homologous cloning which we named as PvCO,PvFT-1 and PvFT-2.The sequences of part of PvCO, full length PvFT-1,PvFT-1 promoter and the ORF of PvFT-2 were analysed. The expression pattern of PvFT-1 was detected by RT-PCR. The result showed that the expressing pattern of PvFT-1 was in accordance with the predicted result from promoter sequence. Further more, we detected the expressions of PvFT-1and PvFT-2 in different tissues of Phyllostachy violascens, the result showed these two genes expressed differently in different tissues. The main conclusions are in following:1. Two homologous genes of CO in Phyllostachy violascens with 80% similarity with Hd1 which contain CCT domain were cloned.We named as PvCO-1 and PvCO-2.2. Four homologous genes of FT which were named as PvFT-1a,PvFT-1b,PvFT-2a and PvFT-2b were cloned in Phyllostachy violascens. We think the PvFT-1a and PvFT-1b were a pair of alleles and PvFT-2a and PvFT-2b were another allele pair. The similarities of these four genes with Hd3a were all higher than 80%.The differences between alleles were just in/del.3. According to the analysis, five light responsive elements and one cis-acting regulatory element involved in circadian control were found in promoter sequence of PvFT-1,. suggesting that it might be regulated by photoperiod and display circadian expressing pattern.4. The expression patterns of PvFT-1 before/after bloom and in 24 hours were detected. Similar to Hd3a, PvFT-1 began to express 20-30 days earlier than flowering. PvFT-1 showed circadian expression pattern with expressing peak at 17:30, which implied PvFT-1 might be regulated by photoperiod.5. Expressions of PvFT-1 and PvFT-2 in different tissues of Phyllostachy violascens were detected, the result showed that PvFT-1and PvFT-2 all expressed in flowering plant while PvFT-1 expressed in leaf and PvFT-2 expressed in blossom bud. 6. For preparing transgenosis, the expression vectors were constructed and transformed into agrobacterium...