The Draft Network Of Flower Regulation In Phyllostachys Violascens Based On RNA-seq And Small RNA-seq

The plant of bamboo which belongs the grass family,has a non regulation,and no periods flowering life phenomena which caused by a special flowering mechanism.In order to searching the mechanism of the flowering regulation in Phyllostachys violascens,we are using the flowering buds of three developing phase（TF₁,TF₂ and TF₃）and the corresponding phases of vegetable buds（TV₁,TV₂ and TV₃）which comes from the same bamboo rhizome for searching.Using high-throughput sequencing,we acquired the genes and miRN A from RNA-seq and Small RNA-seq,which was significantly in searching the flowering mechanism in our species.From the transcriptome,we acquired 317.273 transcripts and 132.678 unigenes,and had the functional analysis such as KOG,GO and K EGG.The differential expressed analysis had sorted out 7266 non-repeated differential expressed unigenes,and the functional analysis of GO and KEGG had analyzed.Two main pathways named plant hormone signal transduction and circadian rhythm-plant which were connected to flowering development were analyzed in details.The results reveal that the genes of AUX1/LAX,CRE1,ARR-B,BSK,CYCD3,NPR1 had a high expression in TF₁ and TF₂,the genes of TIR1,BRI1 had a high expression in TF₁,the genes of PHYA,CHS had a high expression in TF₂,and CTR1 had a high expression in TF₂ and TF₃,the genes of ETR/ERS,EBF had a high expression in TV₁.The sequencing of small RN A acquired 117 mature miRN A which belongs 36 miRN A family,and gained 66 novel miRNA.Conjoint analysis of the two sequencing result,gained 529 non-repeated targets and 193 differential expressed miRN A and their differential expressed targets.The 193 differential expressed miRN A belongs the family of miR172,miR156,miR2118,miR529,miR159,miR164,miR396,miR166,miR169 and miR397.In the 193 differential expressed miRN A and their differential expressed targets,there are 10 differential expressed miRN A（miR159f,miR160a-3p,miR164 c,miR164d,miR166h-5p,miR172 a,miR1873,miR396 g,miR396a-5p,miR396c-5p）whose expression were negative correlation to their differential expressed targets in the six phases.There are 6 differential expressed miRN A（miR395c,miR172d-5p,miR398 a,miR166m,miR167e-3p,miR156a）whose expression were significant negative correlation to their differential expressed targets in the three developing flower buds.And 2 differential expressed miRN A（miR394,miR164a）whose expression were negative correlation in the three developing leaf buds.There are 8 miRNA（miR166a-5p,miR156l-5p,miR169 e,miR529a,miR396c-5p,miR168 b,miR172a,miR172b）who participate in the phase transition from V₁ to F₁.There are 3 miRNA（miR172b,miR172 c,miR2118f）play a role in the phase transition from F₁ to F₂.There are 15 miRN A（miR156a,miR156 k,miR156l-5p,miR319a-3p.2-3p,miR164 e,miR160f-5p,miR156f-3p,miR528-3p,miR5179,miR399 e,miR397a,miR390-5p,miR172 b,miR164d,miR529a）play a key role in the phase transition from F₂ to F₃.Combined the reported flowering time pathway in rice and the sequencing data of RNA-seq and Small RNA-seq,we got the draft flowering regulation network in Phyllostachys violascens.In the flower regulation network,we acquired 101 flowering homologous genes in our species,But the key flower genes such as Ghd7,Ehd1 were not found homologous.There are total 22 differential expressed homologous including Hd1,Id1,Hd3 a and so on.The flowering interaction network model were regulated by the miRNA family of miR172,miR156,miR168,miR169,miR529.