Based On FMRI Technique, The Difference Of Central Response Between Different Body States And Acupuncture At Neiguan And Tongli Was Discussed

Objectives:Evaluate the differences of central response in different body state (chronic stable angina pectoris (CSAP) vs. healthy) after neddling the same acupoints (Neiguan (PC 6) and Tongli (HT 5)) to provide support for the multiple indications of the same acupoints.Methods:1 Study on the differences of brain activities in chronic stable angina patients and healthy subjects:CSAP patients and healthy subjects of 18, respectively, were included. Demographic data of each participant were collected. Each subject underwent a resting state functional magnetic resonance (fMRI) scan. FMRI data were analyzed using regional homogeneity (ReHo) method and Statistical Parametric Mapping software of version 8 (SPM8) on Matlab platform, to evaluate the differences of brain activitis in chronic stable angina patients and healthy subjects. Threshold was set as P< 0.005 (uncorrected) and cluster> 5.2 Study on the differences of central response in CSAP patients and healthy subjects after needling the same acupoints:A total of 8 CSAP patients and 18 healthy subjects were treated with acupuncture for 2 weeks, with Neiguan (PC 6) and Tongli (HT 5) needled. Each subject underwent 2 resting state fMRI scans, one before acupuncture treatment and one after acupuncture treatment. For the patients, the number of attacks during the last 2 weeks were recorded before and after acupuncture treatment; Seattle Angina Questionnaiire were used to assess the symptoms of before and after acupuncture treatment. FMRI data were analyzed using regional homogeneity (ReHo) method and SPM8 on Matlab platform, to evaluate the of central response in CSAP patients and healthy subjects after needling the same acupoints. Threshold was set as P< 0.005 (uncorrected) and cluster> 5.Results:1 Differences of brain activities in chronic stable angina pectoris patients and healthy subjects:Compared with healthy subjects, chronic stable angina patients showed increased ReHo in multiple brain areas, including left lingual gyrus (BA 19), right inferior temporal gyrus (BA 37), right middle temporal gyrus (BA 21), right parahippocampus, right transvers temporal gyrus (BA 41), left lentiform nucleus (putamen), right insula (BA 13), right thalamus (pulvinar), right ventral anterior cingulate cortex (BA 24), right posterior cingulate cortex (BA 30), left parahippocampus (BA 37), right parahippocampus (BA 36), left orbitofrontal cortex (BA 11), left inferior frontal gyrus (BA 47), bilateral dorsolateral prefrontal cortex (BA 9), left semi-lunar lobe, and showed decreased ReHo in multiple brain areas, including bilateral precuneus (BA 7/19), left postcentral gyrus (BA 40), left inferior parietal gyrus (BA 39/40), right superior temporal gyrus (BA 13/38/39), left superior temporal gyrus (BA 22), right middle parietal gyrus (BA 37), left middle temporal gyrus (BA 21), bilateral dorsolateral prefrontal cortex (BA 9), right precentral gyrus (BA 6), right thalamus (ventral anterior nucleus), right caudate nucleus (caudate head), left parahippocampus (BA 27), right retrosplenial cingulate cortex (BA 29), left lentiform nucleus (putamen), cerebellar tonsil and cerebellar pyramis.2 Differences of central response in different body state after needling the same acupints:After treatment, chronic stable angina patients showed increases in left superior parietal gyrus, and decreases in right postcentral gyrus, bilateral thalamus, bilateral insula. However, these changes did not show in healthy subjects.Conclusions:1 Compared with healthy subjects, chronic stable angina patients showed abnormal increased or decreased ReHo in multiple brain areas.2 Compared with healthy subjects, chronic stable angina pectoris patients showed more extensive responses in paimatrix, orientation network, emotional network. And the responses are diseases targeted. However, this characteristic did not show in healthy subjects. Our results suggested different body states response differently to acupuncture at the same acupoints. Our findings might reveal some mechanism underlying that the same acupoint can treat different diseases.