Study On The Quality Of Traditional Chinese Medicine And The Number Of Daily Use Of Pharmacodynamics

The effect of traditional Chinese medicine is the common result of multi components, testing one or more index components, can not fully measure the quality of medicine. pharmacokinetics of an ingredient can not reflect the overall efficacy of traditional Chinese medicine.Therefore, it can not develop a medicine regimen by one or more ingredients pharmacokinetic. In order to supplement the deficiency of chemical analysise, seek new breakthroughs of medicine quality analysis method. We select Cordyceps sinensis as model drug, establish the quality control method of traditional Chinese medicine based on not only chemical analysis, but also biopotency measurement from the view of the biological activity evaluation to characterize the drug release in vitro objectively of traditional Chinese medicine. While selecting Fufangbiejiarangan tablet and Canhuang tablet as model drug for daily administration times study. The biopotency measurement is applied to the study. Combine pharmacodynamics and pharmacokinetics to establish a new model of daily administration times that reflect a holistic view of traditional Chinese medicine.In this study, Cordyceps sinensis was pulverized to a particle size of100to150mesh,150to200mesh,200to300mesh,>300mesh, respectively, to prepare different grinding degree powder sample. The in vitro dissolusion of adenosine was measured at different time. Meanwhile, the powder sample was intragastricly administered to rats, and pharmacodynamics approach was adopted to study the inhibited effect of medicated serum on HSC-T6proliferation. The results showed that the accumulative dissolution rate of Cordyceps sinensis of200to300mesh in vitro was higher than that of other meshes. Pharmacodynamics study showed that medicated serum could significantly inhibit HSC-T6cell proliferation. The AUC of HSC-T6inhibition kinetics of medicated serum that rats were giwen Cordyceps sinensis of200to300mesh was significantly higher than that in other groups. Therefore, the in vitro dissolusion and pharmacodynamics method can be used for reasonable particle sizes study of Cordyceps sinensis on anti-hepatic fibrosis, and200to300mesh is the optimal particle size.In order to study the rational daily administration times. Fufangbiejiaruangan Tablet was drenched to rats with different daily administration times. Blood was colleceted in two tubes from the veins behind the eye sockets at each time point was used to study the effect on HSC-T6inhibition in vitro and pharmacokinetics of peoniflorin in rats, respectively.Then analysis the relationship between pharmacodynamics and pharmacokinetics to develop a reasonable dosing regimen of Fufangbiejiaruangan Tablet in the treatment of liver fibrosis in chronic hepatitis. The results showed that there was good correlation between pharmacokinetics of peoniflorin and the pharmacodynamics of the effect on HSC-T6inhibition in vitro. The AUC of pharmacokinetics and the HSC-T6inhibition kinetics when administrated2times a day were significantly higher than that administrated1or3times. This study suggests that it is more appropriate to administrate2times a day when Fufangbiejiaruangan Tablet is used for anti-liver fibrosis in chronic hepatitis.To study the rational daily administration times of Canhuang Tablet for treating jaundice in rats based on pharmacodynamics/pharmacokinetics model. Rats were modeled by orally administrating4%1-Naphthylisothiocyanate (75mg/kg). After48h, Canhuang Tablet was orally administrated with different daily administration times. Blood was collected from the orbital sinus at different intervals after first administration, and carry out the bile duct ligation at the same time. then we investigated the levels of liver enzymes and bilirubins using these processed blood sample. Bile was collected hourly, and HPLC were used to determine the concentration of berberine in the bile at the same time. Finally, we obtained the time-effect curve and time-dose curve. Pharmacodynamics results show that the total bile within10h of the rats taking Canhuang Tablet once a day were1.32and1.47times higher, respectively, compared with the groups of rats taking Canhuang Tablet twice and thrice a day. and the five biochemical indexes(TBIL、DBIL、ALT、AST、 ALP)of the rats taking Canhuang Tablet once a day decreased more fast than the groups of rats taking Canhuang Tablet twice and thrice a day.The results also showed that there was good consistency between pharmacokinetics of berberine and the pharmacodynamics of the effect on liver enzymes and bilirubins in vivo. AUC0→t of berberine and the cholaneresis and retro-jaundice kinectics when administrated once a day were significantly higher than that of administrated2and3times a day. It is reasonable to take Canhuang Tablet one time a day in the treatment of experimental jaundice. The verification experiment show that, the group administered Canhuang tablet once daily has better results not only in reducing liver enzymes and bilirubins but also in improving pathological changes in liver damage in rat. In summary, Study the efficacy difference of traditional Chinese medicine with different grinding degree based on pharmacodynamics from the perspective of biopotency measurement and in vitro dissolusion to evaluate of the quality of traditional Chinese medicine. This approach allows grinding degree research of traditional Chinese medicine study more scientific and reasonable.The research that Combine pharmacodynamics with pharmacokinetics together to study the rational daily administration times of traditional Chinese medicine will play an important role in elucidating the mechanism of drug action, evaluating the reasonableness of clinical medicine and trying to find out individual differences in terms of the source of proven efficacy. The study raise new research ideas for dosage regimen of traditional Chinese medicine in clinical trial, provide more scientific and reasonable theoretical basis for clinical medication of traditional Chinese medicine, making it safety and efficacy.