Effect Of Dihydromyricetin On Immune Organ Growth, Serum Biochemical Papameters And DNA Damage In Hepatocyte Of Broilers

Dihydromyricetin is a kind of plant additive extracts from Teng Cha, It has many functions, such as antioxidiation, antimicrobial and bactericidal effect, anti-inflammatory and analgesic activities, liver-protection effect, prevent alcoholism, anticarcinogenic activity, antihypertensive effect and so on. However, among of the studies of DMY, many of them are on the fundamental pharmacological of human or mice, few of them are on the poultry production. This experiment investigated the safety of DMY by studying the growth performance, immune organ growth, serum biochemical parameters and DNA damage hepatocyte of broilers. This study had two experiments.Experiment 1: 80 1-d-old Arbor Acres male broilers were randomly allotted to 5treatments, each treatment represented by 4 replicates of 4 birds each . Birds in treatmentsI ,II,III,IV,V received basal diets with perfusing the dose of Omg/kg-bw, 120mg/kg-bw,240mg/kg-bw, 480mg/kg-bw, 1200mg/kg-bw DMY during 32~34d. In order to investigatethe safty of DMY by studing the immune organ growth and serum biochemical parametersand DNA damage in hepatocyte of 37-d-old and 42-d-old broilers. The results were asfollows: ?There is no significant difference between the trial group and the control groupin the index of bursa and thymus. At 37d and 42d, birds perfused DMY had the trend ofimproving the index of spleen .And the dose of 240mg/kg.bw DMY significantly increase(P<0.05) the index of spleen of 42-d-old broiler. ②The difference between the trialgroup and the control group in serum ALB,TP, GLOB, SGPT, SGOT and γ—GT. were notsignificant. ③The dose of 120mg/kg.bw and 240mg/kg.bw DMY significantly decrease(P<0.01) the tail moment and olive moment of 37-d-old broiler. The dose of120mg/kg.bw DMY significantly decrease (P<0.01) the tail moment and olive moment of42-d-old broiler.Experiment 2: The experiment was conducted using 300 1-d-old Arbor Acres male broilers to investigate the safety of DMY by studying the growth performance, immune organ growth, serum biochemical parameters and DNA damage in hepatocyte of broilers. Birds were randomly allotted to 5 treatments, each treatment represented by 6 replicates of 10 birds each. Birds in treatments I, II ,III,IV, Vreceived basal diets with the addition of 0, 0.025%, 0.05%, 0.1% and 0.25% DMY. The results were as follows: ① Birds fed DMY had the trend of improving the weight gain. The differences among the trial groups were not significant. The addition of 0.05% DMY significantly enhance (P<0.01) the feed conversion ratio. The addition of 0.05% and 0.25% DMY significantly (P<0.05, P<0.01) improve the carcass percentage. During the phase of experiment, birds in all treatments hadno pathological changes on immune organs. ?The addition of 0.05% DMY significantly increased (P<0.05) the index of bursa. but all the additions of DMY did not significantly affect the index of thymus and spleen. The difference between II > IV n Vand control group were not significant (P>0.05). ?There were no significant effect on serum ALB,TP ,GLOB among the additions of 0.025%, 0.05% and 0.1% DMY. At 2Id age, the addition of 0.25%DMY significantly decreased serum TP, however it had no significant differences in serum GLOB. The addition of 0.025% and 0.5%DMY significantly decreased (P<0.05,P<0.01) serum GOT. The additions of 0.25% had no significant differences in serum GOT. There was no significant difference between the trial group and the control group in serum GPT and y—GT.?The addition of 0.025%~0.05%DMY significantlydecrease (P<0.01, P<0.05) the tail moment and olive moment;the addition of 0.1 % ~ 0.25%DMY significantly decrease (P < 0.01) the tail moment and olive moment of 21-d-old broilers, and have no significant differences in tail moment and olive moment of 42-d-old broilers.