| The wheat is one of the most important crops, however, the yield of wheat reduced very serious in world-wide area because of the drought in different degree. The drought has become the most important factor that affect the wheat yield in our country at the present time. Therefore, breeding and selecting the drought resistance varieties is an urgent problem for scoop out the potential fertility of wheat in drought and half-drought area. It is a kind of valid shortcut to make use of tissue culture to select drought resistance asexuality line for drought resistance genetic and breeding. However, there is still lack of specific method to authenticate the drought resistance varieties line in cell level. This research aim is making use of the tissue culture to certain the most suitable concentration of the chemical reagent(polyethylene glycol) and the period when the chemical reagent be put into the culture media for drought resistance varieties authentication of wheat quickly. Then offering proof for selecting drought resistance asexuality line and resources authentication of wheat.In this research there are four varieties of anther and immature embryo of wheat were inoculated in solid medium directly containing different concentration of PEG to induce callus and regenerate the plant. Using the weighing method to built-up growth curve and measure the relative weight of callus, measuring the SOD activity, POD activity and MDA content of callus induced from immature embryo ,further more, statistics of initiating rates and observing the agricultural characters of the regenerated plant to study the difference among the different varieties of wheat growing in the solid media containing different concentration of PEG.(1) If anther and immature embryo of wheat are inoculated in solid media directly containing different concentration of PEG, their initiating rate will change distinctly. 1%-5% PEG can increases the initiating rate of anther obviously, but the function is unconspicuous to immature embryo. Under the concentration of 10% PEG, the varieties of anther and immature embryo with high drought resistance have higher initiating rates than the low drought resistance varieties. 15%,20%,25% PEG can lower the initiating rate of anther and immature embryo sharply.(2) Using the different concentration of PEG to imitate the different degree of water... |
