Study On The Technology Of Rieger Begonia Micropropagation

Using Rieger Begonia leaf as explants, an exsomatic tissue culture in vitro was conducted. Callus with ability for differentiation, adventitious-buds and normal regene plant were obtained. The sorts, concentrations and ratios of organic nutrient, carbon source and plant growth regulator in induction, continue generation and rooting media were investigated by applying the contrast and orthogonal experiments. An acclimating experiment of plant transplant in vitro was carried out, as well .The results showed that the suitable induction medium was MS+ BA1.5~2.0mg/L+NAA 0.1 - 0.2 mg/L + granulated white sugar 30g/L + agar 7g/L. This medium could induce callus and differentiate more adventitious-buds to assist the continue generation. The suitable medium for plant proliferation was MS+BA0.6 mg/L +NAA0.1 mg/L + granulated white sugar 30g/L+ agar 7 g/L. The greater propagation coefficient (6<) and the better quality were achieved. The suitable medium for plant rooting was 1/2MS+NAA0. 3 mg/L + granulated white sugar 30g/L+ agar 7g/L. The rooting rate was 100%. Vermiculite was the optimum medium for Rieger Begonia micropropagation in vitro, the survive rate of the root regeneration plant was 93.8%.The experiments of the sorts and concentration of carbon source indicated that production cost were reduced in case of adding 3% granulated white sugar to the medium. The experiment of adding organic material showed that Gly (3.0 mg/L) ,vitamin Bl (0.8 mg/L), vitamin B6 (0.75 mg/L) , inose presoakin (150 mg/L) and niacin (0.25 mg/L) could assist to improve the proliferation rate of Rieger Begonia.