Stduy On Regeneration System And Tissue Culture Technology Of Begonia Tuberhybrida Vosa

The Begonia tuberhybrida Vosa"Kimjangilhwa" is also named Begonia tuberhybrida Vosa.The foundation of rapid multiprapgation system will play a important role for the future application of transgene and other biotechnology.In the experiment,the contrast test and orthogonality test are applied to study that the effect of different media and matching of hormone concertrations during caius inducing,primary culture,subculture,rooting culture.And plantlets transplanting were carried out,a complete regenerating system of the tissue culture was got in this test.The main results of the experiment as follows:1.Primary culture:Using MS+BA1.5mg/L+NAA0.1～0.2mg/L+ sugar30 g/L+ agar 7 g/L as inducing medium and tender leaf as explants, selecting uncontaminated leaf transferring into flask after 6～8 days,the medium turns to be able to induce callus and more adventitious buds, which grows healthy and strong.This is good for prapgation of Begonia tuberhybrida Vosa with two weeks passing.2.Carbon source kinds and concertrations experiment indicate that adding 3%'s sugar to the medium will get better effect and effectively lower the production cost.Test indicate that adding organic matter to media such as:glycines(3.0 mg/L),VB1(0.8 mg/L),VB6(0.75 mg/L), inose(150 mg/L),nicotinic acid(0.25 mg/L) can raise propagation index of Begonia tuberhybrida Vosa plantlets.3.Subculture:MS+BA(0.8～1.2) mg/L+NAA0.1 mg/L+sugar30 g/L+agar 7 g/L was best multiprapagation medium and can got much propagation times.For production,taking white granulated sugar as carbon source matter will reduce the cost and inhence plantlets quality.4.Rooting culture:macroelements,auxins(NAA,BA,IAA,BA,IBA) had obvious effects for rooting,using 1/2 MS +NAA0.3mg/L +sugar20 g/L +agar7 g/L as culture medium,rooting rate may achieve 100%.5.Piantlet transplanting:hardening-seedling and domestication is an important link during plantlet culture,the transplanting survival percentage of plantslets reaches 89%and grows healthy and strong.