Rieger Begonia In Vitro Propagation Techniques

Study on the Technique system of propagatio in vitro in Rieger Begonia Li Chum Xiii (College of Forestryand Horticulture, Sichuan Agricultural University, Ya抋n,Sichuan,China,625014) ABSTRACT Leaf. stems~ stenisof flower~ leafstalk of Rieger Begonia were used for explants in study of organ culture to establish a rapid technique system of propagation in vitro,and to find out the best explant.. medium culture procedure and effective measures to improve the survival rate of seeding,The results were as follow: Iniation mediuntMS+6-BA0.5mg/L+NAAO. lmg/L can get more adventitious buds,afler 30 days culture. The study of differentiation capacity: From different kind of explant,leal5stem>stem of flowes>leafstalk From different section of leaf,niiddle of leaf >lateral of Ieaf>edge of leaf From different age of the leaI~ midium mature leaf >immature leaf>mature leaf >bud leaf Inoculation model: lay it up in the obverse side of the leaf ,the lower survival rate we get, so lay it up the reverse side better than the obverse sideof the leaf Subcultur medium:MS+6-BAO.O5mg/L, theoretically, the rate of propagation of Rieger Begiona could reach about 7 for 30-40 days. In bottle rooting medium: 3/4MS+IBAO. lmg/L, the rooting rate was about 800/o Out bottle rooting:the bottom of stems was soaked in ABT 700 mg/L solution for 7 seconds, then put into medium (sand: vermiculite: pearlitem=1:1:1).The effection is good, the rooting rate was about 52%. Acclimatization medium(sand:vermiculite:pearlite=l :1:1) the higher survival rate, it is about 61.25%.