| To investigate the toxic mechanism of Thermopsine and Anagyrine on CNS in mice , LD50 of anagyrine and thermopsine were determined in mice.The activities of superoxide dismutase (SOD) and Glutathione peroxidase (GSH- Px) and contents of malondialdehyde (MDA),nitric oxide (NO),Glutamic acid(Glu) of plasma, liver and brain were determined by using TU-1901 uv- spectrophotometry in mice, The results showed that the activities of GSH-Px in plasma and brain in the group taken Thermopsine was significantly lower than that in the control group (P<0.01), in liver was markedly lower (P<0.05) ,the activities of GSH-Px in liver in the group taken Anagyrine was significantly lower than those in the control group(P<0.01), in brain was markedly lower (P<0.05) , have no notable decreased in plasma; The activities of SOD were markedly lower than that in the control group (p<0.05) ,except the activities of SOD in brain in the group taken Anagyrine have no notable decreased ; The contents of MDA were significantly higher than that in the control group (p<0.01),except the contents of MDA in brain in the group taken Anagyrine have no notable increased ,;The contents of NO were significantly higher than that in the control group (p<0.01), except the contents of NO in brain in the group taken Thermopsine were markedly higher than that in the control group (p<0.05); The contents of Glu significantly lower in plasma and in liver , but significantly higher in brain(p<0.01), except in liver in the group taken Thermopsine were markedly lower than that in the control group (p<0.05). The experiment results suggested that the toxic mechanism of CNS in mice on Oxytropis glabra DC is related to Anagyrine and Thermopsine—two kinds of the main toxical components ,they both two can induce the production of oxygen free radicals in organism, decreased activities of SOD and GSH-Px and the increased contents of MDA in main organs or tissues and significantly increased contents of Glu and NO in brain . |
PDF Full Text Request