The Physiological And Biochemical Properties Of Oxytropis Glabra(Lam.) DC. Under Naturally Multiple Stress

The western grassland is an important green barrie for China. For various factors, the degradation, desertification and toxic grass phenomenon appeared in a large area of the western grassland. It takes huge ecological and economicl impact on the western region. The toxic grass can spread quickly and occupied the quality grassland. This takes tremendous loss to livestock industry. Oxytropis glabra (Lam.) DC.is a kind of toxic grass in western China。Based on this phenomenon, we chose the Oxytropis glabra (Lam.) DC. living in harsh environments as the experimental group and set the Oxytropis glabra (Lam.) DC living in greenhouse as experimental group. We measured the Soil data in two groups individually. We found that the soil at experimental group is characterized by low moisture content, salted, lack of nutrients (primarily nitrogen and phosphorus). Compared with control group, the Oxytropis in experimental group are live in a place in multiple stress conditions.The photosynthetic characteristics of Oxytropis under different soil conditions had been measured by LI-6400photosynthetic measurement system. The net photosynthetic rate, light compensation point, of experimental group were respectively:5.86μmolCO2.m-2.s-1,44.7μmol.m-2.s-1; The corresponding data of A were respectively:8.76μmolCO2.m-2.s-1,23.7μmol.m-2.s-1.also, we carried out the measurements of stomatal conductance, transpiration rate and water use efficiency at the same time.To analyze the Oxytropis leaf protein differential proteomics, we got a complete gel spectrum of both by two-dimensional gel electrophoresis and analyze the spectrum by the software Image Master2D Platinum6.0.20differentially expressed protein spots were identified by MALDI-TOF-MS analysis and NCBInr searching, which are eight kinds of photosynthesis-related proteins, two metabolic-related protein; two ATP synthase subunit, they are associated with energy metabolism; an environmental stress response-related proteins; a respiratory chain-associated protein.We hope to get the similarities and differences of the Oxytropis between two groups,by examining their leaf photosynthesis and the leaf protein types and quantities.The explanation of the reasons for its superiority in desert areas from the perspective of physiological mechanisms provides a theoretical basis for part of Oxytropis disaster prevention...