Effect Of Main Toxic Components Of Oxytropis Glabra DC.ON Metabolism Of Oxygen Free Radials And Activities Of P-450 In Rats

In order to study the effects of total alkaloids, main components refined from Oxytropis glabra DC., on the metabolism of oxygen free radicals and, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH- Px) and contents of malondialdehyde (MDA) in plasma, liver and brain of rats were determined by UV spectrophotometry, and the activities of NADPH-cytochrome C(P-450)-reductase in liver microsome were determined by UV spectrophotometry. The results demonstrated as follows: The activities of SOD, GSH-Px in liver and brain in the group taking alkaloids were significantly lower than those in the control group (p<0.01), the activities of GSH-Px in plasma was markedly lower than those in the control group (P<0.05), there were no notable difference in plasma SOD activities between experimental group and control group. The contents of MDA in brain and plasma increased markedly in the group taking alkaloids than those in control group (p<0.01), while MDA contents in liver showed no marked difference between two groups. The activities of NADPH-cyt.C-reductase in the group taking alkaloids were significantly lower than those in the control group (p<0.01). The experiment results implied that the total alkaloid in Oxytopis glabra DC. could induce the production of oxygen free radicals in organism, demonstrated by the decreasedactivities of SOD and GSH-Px and the increased contents of MDA in main organs or tissues after being taken by oral. At the same time, the total alkaloid could deduce the activities of NADPH-cytochrome C (P-450)-reductase, which shows that the liver was injured to some extent by the decreased activities of microsomal enzyme.