Evaluation Of Elite Kenaf Varieties And Analysis Based On ISSR Marker Research

Principal components(PC) of twelve yield and quality characters of 40 kenaf varieties from abroad and our country, which were selected by breeding institutes of our country during the period from "eighth five-year plan"to"tenth five-year plan", were estimated. The varieties were classified according to the scatter plot of the first two PC vectors, the first and third PC vectors. Genetic diversity and relationship of the 30 representive kenaf varieties was evaluated according to ISSR markers, which genomic DNAs were amplified with 17 ISSR primers selected from 80 primers. The 30 kenaf varieties were clustered into different groups according to the similarity coefficient (Nei-li), which represented the genetic relationship of different varieties. The major results are as follow:(1) the first three PCs, which might be regarded as fiber yield component factor(53.44%), fiber and stem weight proportion factor(24.94%) and fiber quality factor(7.69%), account for 86.07% variation among the varieties. Based on the coefficients of the first three PCs, seven elite varieties were identified: Fu Hong 952-1, Fu Hong 2-1, KB11,Fu Hong 992, SCS11-09, KB2, Fu Hong 2 . The coefficients of the first three PCs of KB 11, Fu Hong 992, KB2, Fu Hong 2 were better than others. The result was similar to that of traditional classification. The classification by scatter plot of the two PC vectors was a more direct and simpler method, which was also effect.(2) At the level of D=51.08, all varieties were clustered into one major group, one inferior group and 2 single-variety groups(Fu Hong 952-l,Fu Hong 2). At the level of D=39.12, the major group was clustered into 3 inferior groups and 5 single-variety groups(C2032,BG52-l,Tai Hong 763, Jin Shan Wu Ci, Fu Hong 7).(3) From the UPGMA cluster based on the genetic similarity (GS), at the level of 0.785, 30 kenaf varieties were clustered into one major group , one inferior groupwhich had two varieties:Fu Hong 951 and Fei Zhou lie Ye ,and one single-variety group which had only Jin Shan Wu Ci(chi). The result indicated the three varieties have further relationship with others, which genotypes were more different from others too; the genotypes of most varieties we studied were closer. The study also indicated that the genetic difference between the kenaf varieties of our courtry was relative closer, which might be improved through germplasm innovation. Using the genetic difference to choose hybridizing pararents could breed new elite varieties faster which germplasm renewed each other. ISSR markers can apply abundant polymophism information at molecular level, which is a valid way to study genetic divesity and relationship of kenaf species.