Interspecific Relationship And Genetic Diversity Of Lilium Ssp. By ISSR Molecular Marker

The lily (Lilium spp) is one of most important ormental and medical plants on the world. Genetic variation both among and within species was estimated by means of ISSR markers, in order to make protection strategy and to to make the most of valuable resource. The main results were highlighted as follows:1. Interspecific relationship of fourteen wild species and seven cultivars, including Oriental hybrids and Longiflorum hybrids were analyzed by ISSR. Fifteen primers generated 158 reproducible and clear amplification products(PPN), and polymorphic products were accounted for 98.10% (PPB), The effective number of alleles(Ne)were 1.5125, The Nei gene diversity(He) were 0.3156, Shannon diversity index(I)were 0.4841. By the UPGMA dendrogram,Longiflorum hybrids and Lilium brownii var viridulum have closer relationships. Oriental hybrids have a close relationship with Lilium sargentiae and Lilium davidii, the Lilium rosthornii is far away from the others.2. For Lilium regale population, the genetic diversity was also analyzed with ISSR molecular marker, depengd upon their plant ecological community. There were 65 reproducible and clear amplification products(PPN), The 12 primers were generated 96.92% (PPB) polymorphic products. The effective number of alleles(Ne)were 1.5572, Nei gene diversity(He) were 0.3276, Shannon diversity index (I)were 0.4932. The results by AMOVA analysis indicated that the variation within population account for 82.43% and variation among populations accounted for 17.57%, and the gene flow(Nm)was 5.066. On the UPGMA dendrogram, the samples which in closer distributed spatially, usually appeared in a pattern of closer relationship genetically, which may suggested that the spatial distribution of wild Lilium regale occurred in a"clustered"distribution pattern, within or among populations;we shoule go on to study genetic structure and spatial patterns of genetic variation in plant natural population .3. On the 32 samples of Lilium brownii var viridulum, there were 61 reproducible and clear...