Effects Of APS On Erythrocyte Immunity Of Chickens Infected With IBDV

320 Haline White Chickens, being clinical healthy, were randomly divided into 8 groups, 40 for each group. Group 1 was the control and Group 2 to 8, the experimental groups. Blood samples were taken from heart at 21-day-age and the E-C3bRR, E-ICRR, ERER and ERIR were determined respectively. The values had no statistic difference among 8 groups(P>0.05). The results showed that the determing methods were stable and could be used in the experiment.IBDV antibodies in serum samples of all chickens were negative at 21-day-age. Groups 2 to 8 were infected with IBDV (0.3ml each) at 26-day-age. Group 1 and 2 were not treated with APS, and groups 3 to 8 were injected with APS in chest muscle. Group 3 and 4 were injected at 23 to 25days of age, group 5 and 6, at 26 to 31 days of age, and group 7 and 8, at 26 to 28 days of age. The dosage of APS was 5mg each in groups 3,5 and 7, and lOmg each in groups 4,6 and 8. Blood samples were taken from heart when the chickens were 29,32,35 and 38 days of age respectively, and the E-C3bRR, E-ICRR, ERER, ERIR and the activity of SOD and the contents of MDA in erythrocyte were determined. The results were as follows:l.The E-C3bRR, ERER and the activity of SOD in erythrocyte of Chickens in group 2 were significantly lower than the controls (P<0.05), and the E-ICRR, ERIR and the content of MDA in erythrocyte were significantly higher than the control (P<0.05). E-ICRR between group 1 and 2 were statistically different only at 29-day-age (PO.05). ERIR were not statistically different at all ages (P>0.05). The contents of MDA in erythrocyte were obviously different at 32,35,38-day-age (P<0.05). The results showed that the immune functions of erythrocyte were decreased when chickens were infected with IBDV.2.Compared with group 2, the E-C3bRR, E-ICRR, ERER and the activity of SOD in erythrocyte of groups injected with APS were increased. E-C3bRR, except for at 38-day-age in group 5 and 6, at 29-day-age in group 4, at 29 and 32-day-age in group 7 and 8 were significantly different from group 2 (PO.05). ERER at 29-day-age in group 8 and at 35-day-age in group 6 were obviously different from group2 (PO.05). The activity of SOD in erythrocyte at 29-day-age in group 6 and 8, at 32-day-age in group 5 to 8, at 35,38-day-age ofall groups injected APS were mostly different from group 2 (PO.05).Compared with group 2, ERIR and the content Of MDA in erythrocyte of groups injected with APS were decreased. ERIR, except for at 32-day-age in group 6, at 29-day-age in group 7, at 38-day-age in group 5 were significantly different from group 2 (PO.05). The content of MDA in erythrocyte at 38-day-age in group 3 and at 32,35,38-day-age in group 4 to 8 were obviously different from group 2 (PO.05). The results indicated that APS could improve the immune function of erythrocyte.3.The E-C3bRR, E-ICRR, ERER and activity of SOD in erythrocyte of group 6 were the highest among groups injected with APS. E-C3bRR at 29-day-age in groups 3 to 5, at 32-day-age in all groups treated with APS and at 35-day-age in group 3,4 and 7 were significantly different from group 6(P<0.05). ERIR at 29-day-age in groups 3 to 5 and 8, at 35-day-age in groups3, 7 and 8 were significantly different from group 6 (PO.05). ERER had no statistic significance between group 6 and other groups treated with APS (P > 0.05). The activity of SOD in erythrocyte, except for at 32-day-age in groups 5,7 and 8 and 35-day-age in group 5 and 7, the others were significantly different from group 6 (P<0.01). ERIR and the content of MDA in erythrocyte of group 6, were the lowest in all groups injected with APS. And ERIR had no statistic significance between group 6 and other groups treated with APS (P > 0.05). The content of MDA in erythrocyte had obvious difference between group 6 and group 3 at 35-day-age (PO.05). The results indicated that grup 6 had the most significant effect on the immune function of erythrocyte in chickens among the groups injected with APS.