Effects Of Chinese Angelica On Marrow Hematopoieses And Erythrocyte Immunity In Chicken

Chinese Angelica with sweet and pungent taste, mild quality is the torrefaction root of Angelica sinensis (Oliv.) Diels. It has the efficacy of enriching blood, emmenagogue, acesodyne and lenitive, is used frequently for in clinics. Scholars of internal and external have done very particular research on medicinal effect and clinical use. Chinese Angelica has significant functions on immune and haematogenesis system and better curative effects on anti-inflammation and anti-tumor.Objective: To investigate the effects of Chinese Angelica on the marrow hematopoieses in chicken marrow and erythrocyte immunity in chicken, the decoction of Chinese Angelica was used for better results. Blood index, erythrocyte immunity and hematopoieses in chicken marrow were all studied to investigate Chinese Angelica on immunity and hematinic function and provide academic gist for clinic.Methods: Normal group and blood deficiency syndrome group were douched three different dosages (1mL/kg, 2mL/kg, 3mL/kg) of Chinese Angelica decoction respectively. The blood deficiency syndrome model was made by celiac injection of cyclophosphamide for 6 days (80mg/kg·d). RBC, Hb, PCV, the dropping rate of blood cells, RBC-CR1 and RBC-IC were measured, and compared between normal and blood deficiency syndrome groups which were not douched Chinese Angelica decoction. In order to observe Chinese Angelica decoction and serum with Chinese Angelica decoction on the proliferation of CFU-E, BFU-E, CFU-GM, the technique of ectogenetic semi-solid culture medium of bone marrow hemopoietic progenitor cells was used.Results: Chinese Angelica could notably increase RBC, Hb, PCV and the dropping rate of blood cells (P<0.01) of normal chicken, and make the blood deficiency syndrome chicken which of RBC, Hb, PCV and the dropping rate of blood cells decreased by cyclophosphamide restore to the normal (P<0.05). Chinese Angelica could significantly increase RBC-CR1 of normal group, but could not notably affect RBC-IC (P>0.05) .At the same time Chinese Angelica could make the blood deficiency syndrome chicken which of RBC-CR1 was descent and RBC-IC was raised by cyclophosphamide level (P<0.05). Serum with Chinese Angelica decoction could notably facilitate the proliferation of CFU-E, BFU-E and CFU-GM, but Chinese Angelica decoction without serum could not promote the proliferation of CFU-E, BFU-E and CFU-GM. So Chinese angelica which has the proliferation on hemopoietic progenitor cells of marrow accelerates the proliferation of hemopoietic progenitor cells indirectly through hemopoietic factor.Conclusion: Chinese Angelica has the obvious proliferation on hemopoietic progenitor cells of marrow and enhances the erythrocyte immunity. Consequently Chinese Angelica strengthens the immunity and brings into play in anti-tumor. This research provides a valuable basis for clinical application of Chinese hematinic herbs and a new hematinic theory of Chinese medicine.