Development And Clinical Effect Of Film - Forming Dairy Cow Nipple Bathing Agent

Mastitis can cause great harm to dairy industry, and the prevention of this disease is especially important. Teat dipping with disinfectant is a simple and effective means for prevention and control of mastitis, but with a weak point of short effective time. A film-forming teat dipping could solve this problem, and in this study, we developed a medication of this kind and explored the stability and clinical effect of this medication. The study included the following experiments:1. A survey of mastitis in cowsThe clinical mastitis in cows was surveyed, and 8.5% in 201 cows was detected positive, with the incidence of recessive mastitis being 38.9%; The incidence of recessive mastitis was 18.7%,15.6%,19.9% and 20.2% for forward left, forward right, left back and right back udder region respectively. The result indicated that recessive mastitis was an important problem in the dairy farms.2. Preparation of the film-forming teat dipping medicationTake proper amount of distilled water with 5% PVA and stir, after fully swelling, put them in 85℃ water bath pot, then drop to normal temperature, add 5% plasticizer,1.0% emulsifier, 0.6% intensifier with absolute ethyl alcohol dealt and stir again; finally add 5% PVP-I and stir again, and drop to normal temperature.3. Stability of the film-forming teat dippingThe color of the film-forming teat dipping was brown, the texture was uniform, and its pH was 5.5-6.5; after 30 min of centrifugation, there was not stratified phenomenon. The trial of skin stimulation test showed that the medication had no stimulation to nipple skin, and could be used in clinical experiment.4. Disinfection of the film-forming teat dippingField test showed that the film-forming teat dipping can kill the bacteria of cow nipple skin, with a sterilization rate of 99.9% and a killing index greater than 104. There was no significant difference on milk yield, milk protein, milk sugar, butterfat rate and non-fat solid. It can inhibit the activity of pathogens of cow mastitis.5. Clinical effect of the film-forming teat dippingIn this study, we produced one type of film-forming teat dipping agent and evaluated the application effect in dairy cow. The results showed that the incidence of recessive mastitis cows dropped to 18.3% from 41.6% after the use of the product, with significant difference from control. This preparation could reduce the positive rate and strength of subclinical mastitis, and lower the milk somatic cell counts. Compared with the control, the trial group could reduce the incidence of subclinical mastitis by 11.2%, with no significant difference on milk production. The film-forming teat dipping had a good effect on the control of subclinical mastitis in dairy cattle.