Study On The Epidemiologic Investigation Of The Cow Subclinic Mastitis In Weifang Area And The Development Of A Teat-Dipping Preparation

The subclinic mastiffs occurs frequently in dairy cows. This disease can seriouslyreduce the production and the quality of milk, and cause great loss to the dairy industry.The aim of this study was to find an effective and environmental friendly measure forcontrol subclinic mastitis in cow. This study was conducted in four sections.First, a number of experiments were conducted to detect the subclinic mastiffs in theselected cows with LMT diagnostic reagent of mastitis, somatic cell count, electricconductivity and pH measurements, used widely in detection to subclinic mastitis. Thecomparisons among the positive rates detecting with the above methods indicated that therewere not significant differences among those methods. Considering the convenience of theLMT diagnosis, epidemiologic investigation in WEIFANG" area with LMT diagnosticreagent was also conducted.Eight hundred and forty-three heads of dairy cows in eight different diary farms inWEIFANG area were selected to determine the subclinic mastiffs with LMT diagnosticreagent. The result shows that the positive rate of subclinic mastitis in these farm was52.31%, 67.86%, 68.67%, 63.85%, 78.64%, 73.75%, 68.75%and 80%,respectively,which were between 52.31% and 80%. Comparing the positive rate of farm A with that offarm B, C, D and Farm E, F, G, H, the variances were notable. Comparisons among thepositive rate of the subclinic mastitis to the cows of different productive number indicatedthat the longer time a cow produces the higher positive rate the disease incidence was.From the comparisons between farms employing different management strategies, it can beconcluded that the management style can affect the incidence of the subclinic mastitis ofCOWS.In order to determine the effect of teat-dipping preparations in inhibiting bacterium,three of the main kinds of pathogenic bacteria: Staphylococcus aureus, Streptococcusagalactiae and Eschrichia coli were isolated. The isolating rate of these bacteria was 27.88%,11.51% and 7.69% respectively. And further work in the other seven farmsindicated that these three bacterial species existed widely in the milk from the ill cowsuffering subclinic mastitis in WEIFANG area.Teat-dipping preparation named "YIJIFU" was developed in this study, and a seriesexperiments including in vitro inhibitory effect test, the practical disinfective effect test,side effect observation and the clinical effect test, were conducted to evaluate the quality ofthis medicine. The result shows that this liquid can inhibit Staphylococcus aureus,Streptococcus agalactiae and Eschrichia coll. After 10 minutes or 4 hours teat-dipping with"YIJIFU ", the rates of inhibition to both bacteria were 100%. This shows that "YIJIFU"can offer an effective disinfection to the teats and its surrounding skin, and this effect canbe maintained for more than 4 hours. "YIJIFU" has no irritation, no corrosion, no injury tothe skin of cow's teats, and it can protect the skin effectively. The clinic effect test showsthat dipping with "YIJIFU" can effectively combat the subclinic mastitis of cow withoutcausing any decline of milk production.The result of this study indicates that the incidence of subclinic mastitis of cow inWEIFANG area is between 52.31% and 80%. Staphylococcus aureus, Streptococcusagalactiae and Eschrichia coli exist widely in the diary farm in this area. Teat-dipping canreduce the incidence of subclinic mastitis of the dairy cows. Thus, it is an effective andenvironmental friendly control method to this disease.