Study On The Extraction And Purification Of Actinidia Leaves

Flavonoid widely exists in each parts of the plants, especially in the parts of flowers, leaves.Plant flavonoids have anti-bacterialanti-inflammatory, anti-aging, antioxidant, the treatment of cardiovascular disease, lipid and other physiological pharmacological activity.In this paper, we take the actinidia leaf as the object of study. the ultraviolet spectrophotometric method was used to determine the ation total flavanone content of the kiwi fruit leaf.we has studied the process of ethyl alcohol backflow law extrac the total flavanone from the kiwi fruit leaf. as well as the separation purification kiwi fruit leaf flavanone method. and uses the HPLC to compare before and after the purified content of kiwifruit leaves flavonoids.In the study of ethanol extraction of total flavonoids of actinidia leaves,we take the ethyl alcohol volume fraction the extraction time,the temperature,the material fluid ratio,and the extraction pH for the investigated factors as the the single factor condition research. on the base of the single factor condition.the orthogonal experimental study conducted to determine the optimum extraction conditions.we determined the best extraction condition is: the determination best extraction condition is:By 70% ethyl alcohol, according to 1:60 material fluid ratio, in the pH7 solvent,80℃; the backflow extract for 3 hours, come to an extraction of flavonoids To:12.10mg/g. Extraction of factors affecting the strength of the order is:the material fluid ratio> extraction temperature> ethanol concentration> the solvent pH.In this paper,we take rutin as the standard of the kiwi fruit total flavanone, used the static test and dynamic test methods, has separately studied the kiwi fruit flavanone on different resin adsorption and the desorption performance. Through the static adsorption and the desorption contrast experiment, from five kinds of macroporous resins.we found the larger adsorption and desorption rate of D101 type and LD601 macroporous resin for separation of flavonoids extract of kiwi fruit adsorbent, dynamic column chromatography Implementation of the Law Separation. The results showed that:five kinds of macroporous resin adsorption of flavonoids have basically reached a balance of about 1h, within solvent pH2～4 resin adsorption of flavonoids and stability, dynamics data fitting result conforms to the double constant equation, the correlation coefficient achieved above 0.94,we used the the Freundlich adsorption isotherm and the Langmuir adsorption isotherm to carride on the thermodynamics data and found that both of the correlation coefficient is above 0.93, AHO less than 0. the adsorption is an exothermic process. Kinetic experiment to determine the D101 the best resin and LD601 resin concentration on the sample were 60μg/mL and 200μg/mL, the sample rate are 2mL/min, desorption agent were 40% volume fraction, velocity of desorption agent were 4mL/min and 3mL/min. More than 96% absorption rate, desorption rate is above 95%.This article also studide on the purification of the total flavanone from the kiwifruit extract. Take D101-type and the LD601-type macroporous adsorbent resin as the absorbent, the best dynamic adsorption and the desorption condition of the kiwi fruit flavanone on two kind of resins as the operation parameter, uses the dynamic column chromatograph to carry on the kiwi fruit to extract the total flavanone separation purification, the highly effective liquid phase chromatography examination purifies the effect. The research discovered the D101 resin and the LD601 resin have the good separation purification to the kiwi fruit leaf total flavanone.After the D101 resin purification the total flavanone content 11.86% enhances from the original stock to the 24.18%, LD601 resin enhances to 32.258%, and remove some other ingredients, impurity content decreased.