| Chemiluminescence (CL) is defined as the production ofelectromagnetic (ultraviolet, visible or near-infrared) radiation as a result of a chemicalreaction. The process by which CL is generated is as same as that for photoluminescen-ce (fluorescence and phosphorescence) except that the former requires no lightexcitation source. CL is now considered as a very sensitive analytical method and hasbeen applied to different fields such as life sciences, geological analysis, pharmacology,clinical medicine, environmental molecular biology detection and nanometer materialanalysis etc. because of its high sensitivity, low detection limits, wide linear ranges,measure device simple and apt to realize automation advantage. In recent years, it isfound that when injecting some substances into reaction mixture after the finish ofseveral CL reactions, such as potassium permanganate-luminol, potassiumferricyanide-luminol Ce(Ⅳ)-Sodium sulfite system. Such phenomenon is named as apost-chemiluminescence (PCL) phenomenon and the corresponding chemiluminescen-ce reaction as a PCL reaction. We found that many kinds of metal organic matters canproduce PCL phenomena in NBS-luminol system.This thesis contains a review and research report. In the review, the chemilumines-cence systems in which NBS was used as the oxidant were summarized. And the reviewgave emphasis on the CL mechanism and analytical application of several main reactionstyles. In the research report, extensive research was done on NBS-luminol system. Wefound that many kinds of matters can produce PCL phenomena in this system, andoptimized the condition of reactions at the same time, carried on the preliminarydiscussion to their mechanisms, set up new methods to determine piroxicam, meloxicamand halcinonede, thus structure the new system that PCL of NBS-luminol from theoryand practice.The research section includes three parts.Part one, a new PCL reaction was observed when piroxicam was injected into thereaction mixture after the finish of CL reaction of NBS and luminol. The possiblereaction mechanism was proposed based on the studies of the CL kineticcharacteristics, the CL spectra, fluorescence spectra and other experiments. A new flowinjection CL method for the determination of piroxicam was established based on the PCL reaction. The relative standard deviation (RSD) for the determination ofpiroxicam was 1.2％(n=11, c=2.0×10-6g/ml). The CL intensity responded linearly tothe concentration of piroxicam in the range 1.0×10-7～1.0×10-5g/ml (r=0.9991). Thedetection limit was 4×10-8g/ml. The method had been applied to the determination ofpiroxicam in tablets with satisfactory results.Part two, a new PCL reaction was observed when meloxicam was injected intothe reaction mixture after the finish of CL reaction of NBS and luminol. The possiblereaction mechanism was proposed based on the studies of the CL kineticcharacteristics, the CL spectra, fluorescence spectra and other experiments. A new flowinjection CL method for the determination of meloxicam was established based on thePCL reaction. The relative standard deviation (RSD) for the determination ofmeloxicam was2.3％(n=11, c=6.0×10-6g/ml). The CL intensity responded linearly tothe concentration of meloxicam in the range 1.0×10-6～8.0×10-5g/ml (r=0.9995). Thedetection limit was 1×10-7g/ml. The method had been applied to the determination ofmeloxicam in tablets with satisfactory results.Part three, It was found that the CL emitting from the reaction of luminol withN-bromosuccinimide periodate in alkaline solution could be strongly enhanced byhalcinonede. Based on this phenomenon, a new chemilumines-cence method for the determination of halcinonede by flow injection technique wasdeveloped. The CL intensity responded linearly to the concentration of halcinonede inthe range 1.0×10-9～1.0×10-7g/mL. The detection limit was 2.9×1010 g/mL. The relativestandard deviation (RSD) for the determination of 4.0×10-8 g/mL Halcinonede was3.0％(n=11). The method has been applied to the determine halcinonede successfully. |
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