| Chemiluminescence (CL) is defined as the production of electromagnetic (ultraviolet, visible or near-infrared) radiation as a result of a chemical reaction. The process by which CL is generated is the same as that for photoluminescence (fluorescence and phosphorescence) except that the former requires no light excitation source. CL is now considered as a very sensitive analytical method and applied to different fields such as life sciences, geological analysis, pharmacology, clinical medicine and environmental molecular biology detection etc. because of its high sensitivity, low detection limits, wide linear ranges, measure device simple and apt to realize automation advantage. In recent years, Lv etc. report that it is found when injecting some substances into reaction mixture after the finish of several CL reactions, such as potassium permanganate-fluorescence reaction and potassium permanganate-luminol reaction can cause a new chemiluminescence reaction. Such phenomenon is named as a post-chemiluminescence (PCL) phenomenon and the corresponding chemiluminescence reaction as a post-chemiluminescence reaction. PCL was found when injected some metals and many organic compounds in potassium ferricyanide-luminol reactionThis thesis is made up of the review and research paper, analytical application of luminol system in the past ten years was summarized in review part; we introduced emphatically their analysis applications and relevant mechanism. In research paper, extensive research was done on luminol - potassium ferricyanide system, we found that many kinds of metal ions and organic matters can produce PCL phenomena in this system, optimized the condition of reactions at the same time, carried on the preliminary discussion to their mechanisms, set up new methods to determine codeine phosphate, phenothiazine medicine, berberine hydrochloride, thus structure the new system that PCL of luminol- potassium ferricyanide from theory and practice.1 .Post-chemiluminescence behaviors of Ni2+, Mg2+, Cd2+ and Zn2+ in potassium ferricyanide-luminol reactionA new chemiluminescence (CL) reaction was observed when Ni2+, Mg2+, Cd2+ orZn2+ was injected into the reaction mixture after the finish of CL reaction of alkaline luminol and potassium ferricyanide. This reaction is described as PCL reaction. The possible mechanism for PCL reaction was proposed through the studies of the CL kinetic characteristics, the CL spectra and other experiments. The experimental conditions of the CL reactions were optimized and the feasibility using the reaction to analyze these metal ions was evaluated. The PCL reaction method allows to determine 1×10 (-7)- 8×10-6 g/mL Ni2+, 3×10-6×10-4 g/mL Mg2+, 8×10-7 - 1×10-4 g/mL Cd2+and 2×10-42×10-3 g/mL Zn2+with the detection limits of 4×10-8 g/mL, 1×10-6 g/mL, 3× 10-7 g /mL, 8 × 10-5 g/mL, respectively.2. Determination of phosphorous acid codeine, phenothiazine medicines and berberine hydrochloride in potassium ferricyanide-luminol reactionA strong CL signal was detected when phosphorous acid codeine, phenothiazine medicines (fluphenazine hydrochloride, perphenazine, trifluoperzine hydrochloride, thioridazine hydrochloride, imipramine hydrochloride, chlorpromazine hydrochloride), berberine hydrochloride was injected into the reaction mixture after the finish of CL reaction of alkaline luminol and potassium ferricyanide. Based on this, optimizing the experimental conditions of the PCL reactions and establishing the methods using the PCL reactions to analyze these organic substances. The PCL reaction methods allow to determine 8.0×10-8 1.0×10-5 g/mL phosphorous acid codeine, 5.0×10-82.0×10-5g/mL perphenazine, 1.0×10-6 - 1.0x10-4 g/mL fluphenazine hydrochloride 1.0×10-61.0×10-4 g/mL trifluoperzine hydrochloride, 2.0×10-8 8.0 ×10-6 g/mL thioridazine hydrochloride, 1.0 × 10-7-1.0 × 10-5 g/mL imipramine hydrochloride, 4.0×10-8 - 4.0 ×10-6 g/mL chlorpromazine hydrochloride and 4.0×10(-7)2.0×10-5 g/mL berberine hydrochloride. With the detection limits of 3×10-8 g/mL phosphorous acid codeine, 2×10-8g/mL perphenazine, 3×l0-7g/mL fluphenazine hydrochloride, 3×10-7g/mL trifluoperzine hydrochloride, 8×l0-9g/mL thioridazine hydrochloride, 3×10-8g/mL imipramine hydrochloride, 1×10-8g/mL chlorpromazine hydrochloride and 1×10-7g/mL berberine hydrochloride, respectively. The relative standard deviations for phosphorous acid codeine, phenothiazine medicine and berberine hydrochloride in 11 repeated measurements are all less than 3%. The methods developed have been applied to determinate phosphorous acid codeine, phenothiazine medicine and berberine hydrochloride in tablets with satisfactory results. By study in detail, the possible reaction mechanism is proposed in potassium ferricyanide-luminol system. |
