| Chemiluminescence(CL) analysis method has been used actively in the fields of clinical medicine, pharmacy, food safety and environmental detection because of its advantages of high sensitivity, wide linear range, relatively inexpensive equipment and rapid analysis. Metal nanoparticles have large specific surface area, size effect and good chemical reaction activity and other characteristics. It combined with traditional CL reaction system, which broke the situation of the CL reagents only for small molecules and greatly widen the application range of CL analysis. So it has became a hotspot for analysis of workers.This thesis included two parts. the first part was a review which includes the definition, principle, characteristics of CL analysis and common liquid CL system as well as the characteristics, preparation methods of metal nanoparticles and their application in liquid phase chemical luminescence progress, also, it pointed out the problems and research direction of the existing CL system in terms of actual samples especially in the pharmaceutical analysis. The second part was the research report, it reported that the noble metal silver nanoparticles was introduced into the Luminol-K3Fe(CN)6, Luminol-H2O2, Luminol-KMn O4 CL system, which combined with flow injection analysis technology, On the basis of the optimized detect conditions, the lansoprazole, idebenone, piperazine ferulate, mifepristone and other drugs were detected by a new CL detection methods, which were applied to analyses drug content in real samples. As the same time, the possible mechanism of the CL reaction was discussed. The main contents were as follows:1. A flow injection CL method for the determination of lansoprazole was proposed based upon the phenomenon that lansoprazole can obviously enhance Ag NPs-Luminol-K3Fe(CN)6 CL system. Under the experimental conditions, a linear relationship was obtained between the CL intensity and the concentration of lansoprazole over the range from 3.0×10-3~1.5 mg/L with a detection limit of 2.0×10-4 mg/L(3σ), the `relative standard deviation was 1.5% for 3.0×10-2 mg/L lansoprazole solution in 11 repeated measurements. The proposed method was applied to the analysis of lansoprazole in tablets and human serum, the recovery was between 98.0%~102.5%.2. Under alkaline conditions, idebenone on CL system of Ag NPs-Luminol-H2O2 based on a significant inhibitory effect, combined with flow injection analysis technology, build a new CL method to detect idebenone. The CL intensity was correlated linearly with idebenone concentration in the range of 2.7×10-2~60.0 mg/L with a detection limit of 4.4×10-3mg/L, the relative standard deviation was 2.1% for 0.1mg/L idebenone. The proposed method has been applied to the analysis of idebenone in tables and human serum, and the recovery was in the range of 97.5% ~104.0%.3. Based on the enhance CL of luminol-K3Fe(CN)6 system by silver nanoparticles, but piperazine ferulate could inhibit the CL signals significantly, a flow injection method was developed for the determination of piperazine ferulate. Under the optimized experimental conditions, the linear range of piperazine ferulate detection method for the 8.0×10-3~1.0mg/L, the detection limit was 6.8×10-4 mg/L(3σ), relative standard deviation is 1.2%. The method has been applied to the determination of piperazine ferulate tablets and human urine, the recovery rate was 95.0 %~101.7%.4. The experiments indicated that silver nanoparticles could greatly enhance the CL intensity of the luminol-KMn O4 system in sodium hydroxide media, and mifepristone could increase the CL signals significantly. Based on this sensitization phenomenon, the establishment of a CL determination of mifepristone and optimize a variety of factors that affect the intensity of CL, under the optimized experimental conditions. The linear range for the determination of mifepristone was 2.0×10-2~10.0 mg/L with a detection limit of 3.8×10-3 mg/L(3σ), the relative standard deviations for 11 repeated measurements of 0.2 mg/L mifepristone was 2.3%. The method has been used for the analysis of mifepristone in commercial tables with recoveries in the range from 97.0% to 102.5%. |
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