Studies On The Bile-acid Compositions Of Several Animal Bile's Elementary Analysis And The Chenodeoxychlic Acid's Abstraction Craft

Chenodeoxycholic acid (CDCA) is one of active principles of anmiles bile. It was reportedthat CDCA inhibits cholesterol's synthesis in liver cells,makes ratio between cholesterol andcholic acid appropriate and has strong cholagogic action. CDCA can dissolve cholesterolcalculus and be used to treat biliary calculi in clinic.This experimental study uses, biochemistry technology and so on the saponification,acidification, extract separates, the purification chenodeoxychlic acid purely from thecommon animal bile, cheaply estimates its purity through the thin layer chromatographicanalysis is about 70% and 80%. This experiment through comparied the craft of severaltraditional processes to extract the Chenodeoxychlic acid to make the correspondingimprovement, first was the method which used ultra filters replaces the traditional means ofeliminate the mixed craft. this craft not only might save the massive organic solvents, butalso might be effective eliminate the macro-molecule matter, for example: the protein and soon. this article through saponified the important attribute to several anmile bile to carry onOrthogonal experimental design and to determine the best saponified craft condition. In orderto simplify the extraction and purification craft, enhances the product purity, thisexperimental study used the ion exchange resin method to extractseparate and purificate thechenodeoxycholic acid, might know after the HPLC chromatogram analysis extract the mainproduct chenodeoxycholic acid's purity is 90.32%.