Isolation And Map-based Cloning Of Albino Mutant Ems15 In Arabidopsis Thaliana

Chloroplast is a very important organelle of plant cells. It also carries out other essential metabolism besides photosynthesis. Chloroplast biogenesis and development are important in green plant development. The biogenesis of chloroplast from proplastid is the prerequisite of photosynthesis and its development requires coordinated expression of the nuclear and the plastid genome. However, this process have not been completely comprehended by now. Using mutants of the model plant Arabidopsis thalianato research the function of the related gene is a primary method in molecular biology. The analysis of albino mutant is an important way to investigate chloroplast biogenesis. We screened a mutant with a albino phenotype from the mutant library that induced by EMS which is a mutagenesis and genetic analysis indicates it is a single locus recessive mutation. Electron microscopic examination showed that the mutant plastids contained little lamellar without integrate thylakoids. Construct the genetic population and localization of EMS15 gene. In order to isolate this gene, we established a set of molecular markers for the first-pass mapping .The EMS15 gene is mapped to the region between FCA6 and F7K2 on chromosomeâ…£. EMS15 gene was finally mapped to the region about 49kb between F28J12(4) and F28A21(7), including 13 genes. Of which there are 3 genes located in chloroplast by prediction. We found the a terminal mutation in EMS15 gene by sequence analysis. So we proposed the EMS15 is responsible for this mutant phenotype. These data should facilitate us to further investigate EMS15 function in chloroplast biogenesis.