| The transgenic cherry tomatoes integrated with HBsAg gene were transplanted in open space. These plants grew up through proper management as well as wild types. Here are some conclusions we achieved in this research:1. HBsAg gene were integrated steadily in transgenic plants showed in PCR results.2. The morphology of transgenic tomato changed sufficently compared to wild type of plants during two-year's planting. The variant leaves appeared in dark green color that were thicker than usual leaves and looked like potato leaves. Obscure leaf margin, fewer lobular, short and rough internode were evident.3. The plant-derived hepatitis B surface antigen had vaccine potency proved by ELISA assay which was also detected in immunochemical experiment.4. The two-dimensional electrophoresis system was established by using 13 cm pH3-10 Immobiline Drystrips.5. The differences of leaf proteomic between wild type and transgenic type were analyzed by two-dimensional electrophoresis. 699 and 545 spots were detected from 2-DE gels and 415 pairs of spots were found by software. Large numbers of different proteins revealed the changes of transgenic leaf proteomic.
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