| A transgenic tomato (Lycopersicum esculentum var. cerasifarm) plant has been studied and its cytological characters, DNA ploidy and protein express variances compared with the wild-type plant were characterized. These results will provide the basis of mutation mechanism. Here are some conclusions we achieved in this research:1. Leaf total chlorophyll content of transgenic plant have increased slightly comparing the control, while the numbers of chloroplasts in the guard cells and palisade tissue in transgenic plant were 1.7 and 1.6 times more than that in control.2. Microstructure of chloroplast was analyzed by transmission electron microscope. The structure of chloroplast in transgenic plantlet showed expansion, and the shape of thylakoids was blurry and incompact.3. Flow cytometry results suggested that the transgenic plant was triploid.4. Using the Two-Dimensional Gel Electrophoresis, we found 368 protein variances. The control gel was served as the reference. In transgenic plantlet, 122 protein spots showed a decrease in their intensity, while other 246 spots were increased.5. 16 protein spots (Value ratio≥2) were identitied successfully. These proteins involved in many biologic functions. |
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