Construction Of The Plant Expression Vector With The Hepatitis B Surface Antigen (HBSAg) Gene And Expression Of HBSAg Gene In Transgenic Tobacco And Tomato Plants

Hepatitis B is a contagious disease that damages the people's health all over the world seriously.It is a main method for most people to inoculate the vaccine against the hepatitis B virus.The hepatitis B surface antigen is an ingredient of HBV envelop protein, which is not contagious and is the effective composition of the hepatitis B vaccine.The hepatitis B vaccine can not be used widely because nowadays the production of hepatitis B vaccine is carried out via yeast expression system,its procedure is complicated,it needs the special equipment for purification and some technicians,therefore its cost is very high.It can be solved this problem for us to utilize transgenic plants to produce the oral hepatitis B vaccine.In this paper,the plant expression vector containing hepatitis B surface antigen gene has been constructed and transformation of HBSAg gene into tobacco and tomato plants has been carried out in order to obtaine transgenic plants.The experiment was performed and the results were shown as follows:First of all, the plant expression vector pBRSAg was constructed,which contained all elements for plant expression and was suitable for transformation via Agrobacteria,such as CaMV35S promoter,both left and right border sequence for T-DNA in Agrobacteria,plant reporter gene gus,plant screening marker gene hpt.Second,the plant expression vector pBRSAg was introduced into Agrobacteria.Third,it was carried out to tansform the HBSAg gene into tobacco plants,transgenic tobacco plants were obtained,the detection was performed and the result indicated that the HBSAg gene was expressed in transgenic tobacco plants. Fourth,the systems of the tomato regeneration and transformation were established and the introduction of the HBSAg gene into tomato plants was performed,the result was shown that the HBSAg gene was also expressed in transgenic tomato plants.These results might provide the theoretical and practical bases for studying the production of oral hepatitis B vaccine ultilizing transgenic tomato plants.