| There are nearly 4000 kinds of cestodes that have been reported from all over the world, and close to half of them have been found parasitizing in fishes. Presently many cestodes of marine fishes haven't been investigated, and the phylogenetic relationships among them are still unascertained. The author try to contribute to solving these problems. The work can be wholly divided into two parts:First: Investigation of cestodes of fishes in Taiwan Strait. The author investigated the frequent marine fishes of Xiamen market from April, 2003 to March, 2005, and gained some cestodes from the fishes, subsequently we identified them carefuLly by light microscope morphological observation together with Scan Electron Microscope photos, in this article we describe 11species, which belong to 3 orders, 5 families and 7 genuses respectively. Of them the author has identified two new species by morphological comparison. Besides we propose to construct a new genus to contain one of the new species that have been found. They are all listed as follows: 1. Paraechinophallus japonicus Yamaguti, 1934 2. Parabothriocephalus gracilis Yamaguti, 1934 3. Parabothriocephalus psenopsis Wang, 2004 4. Anteropora xiamenensis n. sp. 5.Cephalobothrium longisegmentum Wang, 1984 6. Acanthobothrium grandiceps Yamaguti, 1952 7. Acanthobothrium micracantha Yamaguti, 1952 8. Acanthobothrium sp.1 9. Acanthobothrium sp.2 10. Anthobothrium bifidum Yamaguti, 1952 11. Biotobothrium platyrhina n. g. n. sp.Second: The preliminary study in molecuLar phylogeny of cestodes. We first extracted the genomic DNA of the new species, then amplified the SSUrRNA gene by PCR, the amplified gene was cloned into plasmid and sent to be sequenced. (the sequences of this class cestode DNA had never been reported). We added up 12 SSUrRNA gene sequences which came from 8 orders and 12 genuses to align。Then we employed the widely used phylogenetic software PAUP* to construct phylogenetic trees. We used three different construction methods (NJ, MP, ML) of phylogenetic trees. All of them resuLted in the relatively identical outcome. Our resuLts approve the paraphyletic position of Tetraphyliidean. Some species of Procephalidean shouLd be brought into Tetraphyliidea, and they are also in support of the recent amendment (EUZET,1994) of Lecanicephalidea. |
