Research Of Mechanism Of Phenotypic Plasticity And Optimizing Basic Media On Plantlet Propagated In Vitro

Aiming at irrational problems that the number of species micropropagated successfully is fewer and plantlets have lower rooting rate and growth quality occurred in propagation in vitro, we advance the production and differentiation of phenotype of in vitro plant refers not only the cell Totiptency can express, but also form a process that how to generate plastic response to adapt micro-environment. The ability of phenotypic plasticity of in vitro plant is correlated to the growth quality significantly. Focus on how to adapt the particular environment to guide optimizing basic medium in vitro, following results show out:1. Using Arabidopsis thaliana as experimental material, set different treatments on the concentration of N,P,K, a phenomenon present that plantlets can access to reproductive phase and can blossom out and fruit indeed. This indicate that plantlets will generate plastic response to nutrient demand, a contributing and positive effect will turn up on increasing the fitness of plantlets in vitro if nutrient elements compose reasonably.2. Pyrus sinkiangensis Yüis a economical and woody plant which roots difficultly, collecting current research focus that how to optimize the basic medium and taking Pyrus sinkiangensis Yüas experimental material, change the amount of nutrient elements of N,P,K,Ca,Mg at the guidance of nutrient plasticity principles, then two medias which both rooting rate is above 90% are obtained which labeled as S14:620 mgl-1 NH4NO3+740 mgl-1KNO3+40 mgl-1MgSO4+0 mgl-1 Ca(NO3)2 and G1:1769.4 mgl-1 NH4NO3+800.9 mgl-1KNO3+496 mgl-1MgSO4+398.6 mgl-1 Ca(NO3)2.3. In test tube, plant's phenotypic response is the interactive result of"non-natural growth"(eg rooting or proliferating? ) under artifical manipulation and"natural growth"(eg leaves will photosynthesize)under self-development property. Rooting firstly for in vitro plant to survive belongs to"natural growth", therefore, plantlets with higher rooting rate correspond to the excellent adaptive performance. Analyse the nutrient content of plantlets cultured in S12 and S14 that have higher rooting rate above 95% and convert the nutrient data into basic medium, then find out two new medium that rooting rate is above 92% which labeled as S12-2,S14-2.Phenotypic plasticity is foundation of growth and development of in vitro plant, we analyze the plastic change of plantlets to nutrient demand only viewed from optimizing media. Take phenotypic plasticity as guiding principles of in vitro propagation, pay much attention to environmental factors to reform the growth of plantlets, which will supply much efficient theory guidance for increasing and reforming the propagating technology in vitro.