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Study On The Antagonstics Of Probiotics On Helicobacter Pylori

Posted on:2008-09-13Degree:MasterType:Thesis
Country:ChinaCandidate:G YuFull Text:PDF
GTID:2120360215488151Subject:Microbiology
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Helicobacter pylori (Hp) infection is the major cause of chronic gastritis andpeptic ulcer diseases. Routinally combination of antibiotics with other fractions wasused to treat Hp infection. However, this therapyresulted in side effect e.g.resurrection of Hp infection after curing. Thus, approaching new biological therapy,instead of or partially replace of antibiotics therapy is warranted. The aim of this.study was to screen out antagonistic strains against Hp from collections of probiotiebacteria, separate and purify the bioactive substances involved into the antagonismsand characterize the physiochemical properties of those substances. All the approachwas to develop a new nutraeeutical for the prevention and curing of Hp infection. Thewhole research work is described in five chapters.Chapter 1 provides the general introduction of the background of the whole researchwork.Chapter 2 describes the optimization of the antagonistic method against Hp. 33probiotie strains were used to screen out the antagonistic strains against Hp, It wasfound that only the spent culture supematant (SCS) of Bacillus subtilis Km inhibitedHp. Moreover, that SCS had antagonisticeffect on Listeria monocytogenes,Staphylococcus aureus and Pseudomonas aeruginosa.Chapter 3 describes the stability of SCS of B. subtilis Km. It had been shown that theSCS of B. subtilis Km maintained the antagonistic effect again Hp, when treated underpH 2.0-8.0 or heated at<95℃, the antagonistic ability was not affected by thedigest ion of pepsin, trypsin, proteinase K and proteinase E.Chapter 4 describes the separation, purification and determination of the inhibitorycomponent of the SCS. With the.aid of centrifugal filtration, acid gradual precipitation,negative ion exchnge, non-denaturation PAGE and mass spectrum, the antagonisticsubstances were deduced toα-amylose and one hypothetic protein.Chapter 5 includes the conclusion remarks and summary of the whole results.
Keywords/Search Tags:Helicobacter pylori, Bacillus subtilis, probiotics, atagonistics, electrophoresis, mass spectrum
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