Study On The Pollen Morphology And Pollen Development Of Liliaceae

Liliaceae is one of the most important plant species in the angiosperm. To research the germ-plasm resources of Liliaceae is regarded as very valuable at home and abroad. Palynology and reproductive biology of plant are the common methods to research the phylogenetic development of plant.Pollen grains of 8 species of Hosta longipes (Franch.et Savat.) Matsum var., Hosta ventricosa (Salisb.) Stearn, Hosta ensata F. Maekawa, Chlorophytum capense (L.)Kuntze, Hemerocallis citrina Baroni, Fritillaria maximowiczii Freyn, Lilium pumilum DC. and Paris verticillata M.-Bieb. were observed and compared by light microscope (LM).Be based on the result of the studies, some conclusions are drawn as follows: The pollen grains of Liliaceae are generally monocolpate (distal). Generally the side of the grain opposite the colpus is narrow, and the colpus generally extends fully to the end of the grain. The equatorial view of the pollen grains are boat-shaped, elliptic or round, and the polar view are elliptic or round. The sculptures are thin- reticulate, thick- reticulate, granulate- reticulate or foveolate- reticulate.The pollen development of H. longipes (Franch. et Savat.) Matsum and its variation, C. capense (L.) Kuntze and P. verticillata M.-Bieb. were studied by paraffin section and light microscope. This paper briefly describes the progress in microsporogenesis,male-gametogenesis, the type of mature pollen, anther development and tapetum development during the pollen development.The results of reproductive biology were observed as follows: The microsporogenesis of Liliaceae is successive type. The tetrads are mostly isobilateral or tetragonal, and less linear. The pace of pollen development in one anther is basically synchronization, but not going all the way. The mature pollen of Liliaceae is 2-cell or 3-cell type. One species has only one type of pollen, but one genus may have two types. There are usually some abnormal pollen in the anther, but the types and odds are different. The tapetum of Liliaceae is secretory tapetum. The mature anther wall is usually consisted by a layer epidermis cell and a layer endothecium cell, except that some species have two layers endothecium cell. Calcium oxalate crystal, which is raphides, is ubiquitous in the anther of Liliaceae. But the amount, phase and position of distribution are...