Screening And Molecular Genetic Research For Two Novel Arabidopsis Thaliana Mutants Of Pollen And Embryo

To further understand mechanisms that control male gametogenesis and embryo development,we performed an genetic screen for mutations that caused distorted Mendelian segregation in Ac/Ds transposon system in Arabidopsis thaliana.One mutant(ovp1)that displayed defective pollen development and another mutant(eed1) that displayed defective embryo development were focused on.The mutant phenotypes and disrupted gene molecular genetic research were studied.Phenotypic analyses revealed that ovp1 heterozygotes have about 50%aborted mature pollen.Reciprocal crosses and pollen tetrad directly demonstrate that ovp1 is a male gametophytic lethal mutant in the Landsberg erecta ecotype.Ds insertion resulted in At2G01560.Situ hybridization and OVP1_promoter∷GUS analysis revealed that OVP is mature pollen-specific expression.Subcellular localization study demonstrated that the OVP1 is localized to cell nucleus.eed1 displayed 20%embryo defects,and whole stain-clearing technique revealed that the embryo development was arrested before heart embryo stage in eed1. Reciprocal crosses demonstrate that eed1 homozygote is lethal in the Landsberg erecta ecotype.Ds insertion resulted in 3'-end of At2G26120,and we can't obtain eed1 homozygote plant.RT-PCR and promoter-GUS analysis revealed that EED1 is expressed ubiquitously in Arabidopsis plants and strongly expressed in root tip, mature pollen,leaf edge.