Screening Mirosatellite DNA And RAPD Loci Of Mongolian Gerbil And Analyzing Population Genetic Structure

Mongolian gerbil has been applied in many branches of life science, which is thought of as many a functional laboratory animal. But reports about its genetics are a few and population genetic structure is not distinct. So it is important to study the gene diversity of Mongolian gerbil. It is advantageous to understand the genetic structure and population state, to provide the message for breeding, controlling characters and producing management It will also accelerate the standardization and the process of Mongolian gerbil as laboratory animal.Microsatellite loci were screened from rat's and mice's microsatellite loci, RAPD loci of gerbil were come from 50 RAPD primers by PCR. 4 colonies(Bl and B2 of capital university of medical science, Z1 and Z2 of the laboratory animal center of ZheJiang province) came from 2 laboratory animal factories were studied by 13 microsatellite loci and 20 RAPD primers screened. The result shows that allele number is between 1 and 4 average allele number is 2.1538. The average effective allele number is 1.598 and has a little difference in the average allele absolute value, which demonstrates that effective allele number is low and gene frequency of allele is well-distributed. 108 bands of 20 primers are distinct, stable and 18 primers are polymorphic. The length of fragment amplified by PCR is between 200bp and 2000bp. The number of fragment amplified by PCR with single primeris between 2 and 9. The statistical analytical results of microsatellite DNA and RAPD show that 4 colonies are in the Hardy-Weinberg equilibrium state, he average microsatellite DNA loci homozygosity are higher(0.5027~0.6044), the average microsatellite DNA loci heterozygosity are lower(0.3956~0.4973) and Nei's gene diversity and Shannon index of microsatellite and RAPD are lower too. Dendrogram demonstrates the Nei's genetic distance between B1 and B2 is far and the genetic distance between Z1 and Z2 is close. Butthe statistical analytical results only show the significant difference between B1 and Z2As mentioned above, we had detected the method of screening microsatellite loci of Mongolian gerbil from the rat's and mice's, found 8 microsatellite loci. 13 microsatellite loci and 20 RAPD primers can analyze Mongolian gerbil. The statistical analytical results of Mongolian gerbil by microsatellite DNA and RAPD marker are concordant. But the result of microsatellite DNA is directer, whose amount of information is bigger, whose the repeat and stability are better. Genic Variation lies in 4 colonies, genetic diversity is B1>B2>Z1>Z2. 4 colonies are in the Hardy-Weinberg equilibrium state, genetic diversity of Bl is highest among 4 colonies.