Genetic Diversity Of Different Geographical Populations Of Spider Pardosa Pseudoannulata By RAPD Analysis

Fifith-five strains Pardosa pseudoannulata collected from Hunan, Hainan and Yunnan provinces of China distributed in 8 floristic regions were assessed for genetic diversity using the RAPD technique and the correlation with the surrounding environmental factors. Under predetermined optimal reaction conditions,the amplifications gave 84 reproducible and stable fragments ranging from 200 bp to 2500 bp using 10 random primers selected from 50 ones. There were 62 polymorphic fragments and the total percentage of polymorphic loci is 73.8%. The higher percentage of polymorphic loci of eight subpopulations was in Tianding of Changsha City(38.7%) and lower(17. 7%) in Nanxin of Sanya City. The result revealed that there is significant genetic diversity in natural population of Pardosa pseudoannulata which come from different geographical habitants.By Shannon phenotypic diversity index from RAPD data, it was found that 64.24% of molecular variation existed within subpopulations while 35.76% of which among subpopulations. That showed there were high genetic diversity among inter and intra-population. The genetic average distance among eight subpopulations of Pardosa pseudoannulata was 0.2426;the largest was 0.3725 between Ega and Wuzhisan, and the smallest was 0.0753between Nanxin and Danzhou. It also showed the genetic variation existed among the subpopulations.The cluster analysis could classify 55 individuals by SPSS method. It also showed there were some differentiations among subpopulations. From the picture, we can make out which were clustered three branchs. One branch is the Tianding population and the Leifeng population, the other branch is the Shangpa, Yamu and Ega population, the last branchs is the Nanxin, Wuzhishan and Danzhou population firstly. The populations of provinces Hunan and Yunnan provinces assembled one branch first, then did populations in provinces Hainan. The cluster analysis results showed it was related to the geographic distance.Correlation analysis of total 8 populations show a positive relation between genetic diversity and altitude and latitude, but not significant. The genetic diversity and annual average temperature had a significantly negative correlation (P<0. 05). Besides the pesticide influence, correlation analysis of other populations show a most positive relation between genetic diversity and latitude (P<0. 01). It suggested that latitude and the annual average temperature might be one of important factors influencing on the genetic diversity of Pardosa pseudoannulata. It also suggested the use of pesticide will reduce the genetic...