Study Of Different Nutrition Condition On Callus Inducing And Plant Propagation In Sedum Amplibracteatum

The whole plant of Sedum amplibracteatum is worthy of edible, herbal and ornamental. While the plant seed propagation ability is greatly weak, wild source is sparse, and it could be vulnerable to plant diseases and insect pests when the plants were cultured in low altitude area. The paper firstly described domestic and overseas research progress of propagation and chemical components. With wild plant of Sedum amplibracteatum as materials, by single factor and L₉(3⁴)orthogonal design experiment, effects of different nutrition condition on callus inducing, callus differentiation, rooting were studied, and regeneration plant was available, on the purpose of getting regeneration plant and providing with basic theory for chemical component research. The results were as follows:1. April was the best sterilizing time, callus inducement ratio of explants (stem and leaf) reached 100%, and callus growth inducement rate reached 88.57. Stem could be induced callus after 4 days and leaf after 10 days. In other sterilizing time, the measure of soaking with 200mg/L PVP for 15²0min after treatment of 0⁴℃ temperature helped to reduce browning ratio. MS was the optimum medium type.2. 2,4-D induced stem callus firstly than NAA in earlier stage of callus inducement (one week after inoculated), in which callus appear white pellet and delicate structure, while NAA induced leafstalk callus firstly than 2,4-D. In later stage (two weeks after inoculated), callus growth potentiality with NAA in the medium was higher than with 2,4-D, in which callus appear light yellow pellet and puffy structure. The optimum plant hormone concentration that promotes stem and leaf to callus inducement and growth were 2,4-D0.2mg/L, NAA3.0mg/L, 2,4-D0.5mg/L, NAA4.0mg/L separately; 4.0mg/L 6-BA kept callus bloomy fissions and callus appeared green, so greener buds formed, and differentiation ratio reached 75%. Effects of 0.5mg/LTDZon callus inducing and 1.0mg/LTDZ on differentiation were best, and growth inducement rate and differentiation ratio reached 93.27 and 76.19%, respectively.3. Sucrose and edible sugar could promote callus of hyperfunction fission type to information, growth and biology weight increasing, especially 30g/L sucrose, 40g/Lsucrose and 20g/L edible sugar, fresh weight increasing ratio reaching 415.11%, 412.86% and 421.23% respectively, while glucose only induced senescence callus.4. 450mg/LCH and 300mg/LLH could promote callus inducement and growth. Growth inducement rate reached to 90.16 and 86.83, especially 450mg/LCH promote callus to differentiate more green buds, and differentiation ratio reached 83.87%.5. 6-BA and sucrose was the main influence factor of stem callus inducement and differentiation. The optimum callus inducing medium was MS+NAA5.0mg/L + 6-BA1.0mg/L+sucrose 25g/L for leaf, with growth inducement rate reaching to 88.04, MS+NAA3.0mg/L+6-BA 1.5 mg/L +sucrose 30g/L for stem, growth inducement rate reaching 84.43. The optimum callus differentiation medium was MS+6-BA4.0 mg/L +NAA1.0mg/L-f sucrose 40g/L, with differentiation ratio reaching 75%°6. The optimum medium for rooting was l/2MS+NAA2.0mg/L+sucrose 30g/L, with rooting ratio reached 100%, average rooting number was eight, and many roots appeared thinner and longer (about 3.5cm)?...