| The Gesneriaceae family, with few exceptions, is chiefly warm-climate, found in tropical areas. This giant family of some 140 different genus, and approximately 2000 species, includes hundreds of handsome, often velvety-leaved plants with showy wheel-shaped, tubular, or bell-like flowers. The gesneriads are so variable in foliage, texture, growth, and flowering habits that one would have to be a specialist to realize mat all were member of the same plant group.The western hemisphere-Mexico, the West Indies, Central and South America-offer the great wealth in species of gesneriads in cultivation today. Most important of this tropical American group are the Sirmingias, the Columneas, Episcias. Africa is not so rich on species, but from a rather small area in East Africa originate the various Saintpaulia, Which as the "African violet" is easily today the most admired little flowering plant in the home of West countries.Many curious gesneriads come from Asia, amongst them are Aeschynan, Chirita, Hemiboea, Titanotrichum and so on. Fifty six genus, about 450 species have been found in China, and there are 28 genus could be found in China only. Lots of the gesneriads are rarely species, lots of mem are beautiful ornamental flowers.This paper deals with the methods for in vitro conservation of gesneriads. The another purpose of this experiment is to search and demonstrate the high regenerative capacity of leaf tissue of gesneriads. The contamination rate was greatly increased by using a improved surface-sterilized -technology and had more adventitious shoot formation in MS medium supplemented with BA 0.1-0.3 mg/l, NAA 0.1 mg/l. When optimal levels of growth substances, particularly auxin and cytokinins, are utilized, many species of gesneriads can be generated from a single leaf. Today, about 15 species and more than 25 clones of Gesneriaceae plants have been conserved in vitro and propagated. A procedure for the in vitro regeneration shoots via organogenesia of gesneriads is described.
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