Preliminary Study On Cell Culture And Rapid Propagation Of Catharanthus Roseus (L.) G. Don

In this thesis, the callus of Catharanthus roseus (L.) G Don was induced, which used young leaves as explants. The influences of light on callus growth were studied as well as the physiological, biochemical characters and alkaloids accumulation during the growth circles. The results were as follows:1 The optimum medium, which was chosen for the callus induction of Catharanthus roseus, was MS+NAA0.5mg/L+2,4-D0.5mg/L+6-BA2.0mg/L+ sugar3 0g/L+agar7g/L.2 Inthe light and dark, the callus growth circles were about 27 days, and the growth curves looked like "S", which could be divided into lag phase(0~9d), log phase(9~24d)and stationary phase(24~27d). The cells grew more rapidly in the light than that in the dark.3 In the light and dark, the contents of soluble protein, the activities of peroxidase(POD), superoxide dismutase(SOD) and esterase(EST) were studied,and they all showed their changes respectively.4 The alkaloids were accumulated primarily in the stationary phase. The content of alkaloids in the dark was higher than that in the light during the growth circle. The production of alkaloids at 27th day in the dark was much higher than that in the light.5 We can use calli which induced from young leaves to make a study of rapid propagation. There were buds when the calli were cultured for 30 days on MS+sugar30g/L+agar7g/L+6-BA5.0mg/L+NAA0.3 or 0.5mg/L. The optimum medium for buds multiplication was MS+NAA0.1mg/L+6-BA0.5mg/L+sugar30g/L +agar7g/L. And the young plants would be rooted when cultured on 1/2MS+ sugar20g/L+agar7g/L+NAA or IBA if the concentrations of NAA or IBA were under 0.5mg/L, or adventitious roots would be callus again.