The Study On Mutagenic And Mechanism Of The Ion Beam On Arabidopsis Thaliana

By using Arabidopsis thaliana as material, the study had been focused on the ion beam implantation -induced effects and the changes of the configuration. With the RAPD method and the analysis of the POD, the mechanism in molecular biology and physiology were stated.The ion beam implantation induced aberration of Ml in many aspects. Firstly, it was displayed dominantly in the germination rates. The germination rate-dose function curve is down in the first, then up and down at last. Moreover, the injury rates of the nutrient organs (the height of the plants, the lengthen and the width of the leaves) were different when the dose was changed. One of the most evident aspects is the short of the plant lengthen, in the big dose was only 30 percent of the CK. Secondly, the observation of the Arabidopsis thaliana seeds which were irradiated by the ion beam through the SEM found that with the increase of the ion beam dose, the ion beam mechanical erosion degree of the seeds was deep. And there are many holes and gaps in the surface of the seeds in dose. Thirdly, the changes of peroxidase(POD) activity and the analysis of the enzyme spectrum in the morphological different stage in the Arabidopsis thaliana were tested. The result indicated that the relation of dose and the activity was consistent with that of dose and the injury rates of the nutrient organs. Lastly, the RAPD method make the effects of the ion beam irradiation induced the Arabidopsis thaliana DNA clear. And in the molecule level explain the results of the different doses act on the Arabidopsis thaliana DNA and the molecule mechanisms.