Study Of Salt Resistance Mechanism By Gene Transfer Of Radish Peroxidase In Arabidopsis Thaliana

Arabidopsis thaliana，with small genome and short reproductive cycle, has long been used as themode of the molecular biology and genetic studies experimental materials. The peroxidase take not only animportant role in plant growth, development and senescence, but also improve stress resistance in plants.The laboratory has successfully constructed Rsprx1Arabidopsis thaliana. We are committed to researchthe effects of sucrose to raise the ability of Rsprx1Arabidopsis thaliana in salt tolerance.High salt stress mechanism is one of the hotspots of biological research. In order to investigate theeffects of sucrose and peroxidase in the tolerance of Arabidopsis thaliana against high-salt stress, wild typeArabidopsis thaliana including its transgenic, were treated by different concentration of NaCl and3%sucrose in1/2MS medium. The results showed that:1. In the regulation of sucrose, the activity of peroxidase, CAT and APX increased, and the enzymeactivity of Rsprx1Arabidopsis thaliana increased larger. Illustrate that the peroxidase canimprove plant resistance;2. In the regulates of sucrose, the content of anthocyanin, reducing sugar, soluble protein were alsoincreased, and Rsprx1Arabidopsis thaliana exhibit more sensitivity to sucrose, also improved theanthocyanin synthesis;3. In the regulates of sucrose, the K+/Na+ratio decreased, peroxidase improved the plant salt stressability;4. In the regulation of sucrose, anthocyanin synthesis genes and regulation genes were upregulated.Therebefore, the peroxidase gene (Rsprx1) of Chinese red radish improves resistance to salinity inArabidopsis thaliana by increasing the expression of peroxidase gene to increase the peroxidase activity,can be regulated by sucrose, as well as promoting anthocyanin synthesis, thereby increasing the salttolerance of plants.