Primary Research On Sugar-light Interaction To The Anthocyanin Metabolism In Arabidopsis Thaliana By Transfer Radish Peroxidase Gene

Anthocyanins are widely distributed in higher plants, a water-soluble pigment, belonging to theflavonoids, constitute the main pigment of the petals and fruit color are plant secondary metabolites play animportant role in the physiology. Arabidopsis belongs to the long-day plant, its growth and developmentand reproductive affect with illumination time and intensity. This study by changing light conditionsArabidopsis needed for growth, the use of different light intensity and time to study the turn radishperoxidase gene (RsPrx1) Arabidopsis at different concentrations and types of exogenous sugar anddifferent light, has good resistance to provide a new scientific theory, some physiological and biochemicalindices determination to further clarify the regulation of anthocyanin anabolic and catabolic.This study results show that sugar-ray interaction to the vitality of the treatment on the anthocyanincontent of the body turn radish peroxidase genes of Arabidopsis and POD series of antioxidant enzymesand other physiological and biochemical indexes have certain. Of light on anthocyanin synthesis have agreat role to specific induction of anthocyanin accumulation, and anthocyanin content of a sharp increase inthe weak light of a significant difference, indicating that light turn peroxidase RsPrx1gene Arabidopsislines, anthocyanin metabolism has important implications. At the same time, studies show that60mmol/Lsucrose treatment group significantly improved transgenic plants anthocyanins and peroxidase content.High light the plant is a kind of stress, the turn radish peroxidase RsPrx1gene of Arabidopsis thaliana toresist such adversity, the body of POD, CAT activities of antioxidant enzymes increased, thus enhancing thestress resistance of plants, to resist the plant growth environment in the light of adversity. POD isoenzymeanalysis showed that bright light treatment of Arabidopsis POD band intensities in the handling of low lightintensity. Anthocyanin biosynthesis related genes by RT-PCR results showed that the anthocyaninbiosynthesis gene expression was significantly stronger under conditions of high light in low lightconditions,60mmol/L sucrose group anthocyanin synthesis gene expression is stronger than the othertreatment groups. Thus, changing the light intensity with time, and sugar processing Interactions ofTransgenic Arabidopsis peroxidase expression and metabolism of anthocyanins produce a certain influence. Therefore, the light and sugar interaction on growth and development of the transferred peroxidase enzymeRsPrx1Arabidopsis thaliana has an extremely important impact on expression and anthocyanin metabolismby altering the expression of certain genes, thereby affecting the transgenic Arabidopsis peroxidase.