The Relationships Between The Physiological-Biochemical Changes And Ca～(2+)-ATPase During The Peach And Strawberry Senescence

This experiment was performed using the 'Yingqing' peach fruit and 'Chunxing' strawberry to study the changes of the main physiology-biochemistry characteristics and the microsome membrane Ca +-ATPase activities during the fruits ripening. The effects of ethylene, trifluoperizine (TFP) +ethylene and verapamil (Ver) +ethylene treatments on the above changes were discussed. The objects of the thesis may further explore the role of calcium messenger in the ethylene signal transduction, and provide some new theoretical basis for further research of the microsome membrane Ca2+- ATPase during the fruits ripening.The results of the study were as follows:The postharvest respiratory rate of 'Yingqing' peach had the character of climateric fruits,but it was not typical climateric fruits. The respiratory rate reached its peak after the fruit ripening, and before the ethylene production rising. The respiratory rate of 'Chunxing' strawberry decreased gradually after harvest, but it increased in the end of the fruits senescence. Therefore, 'Chunxing' strawberry was one late-respiration peak type fruit.The ethylene production of the two fruits emerged peaks after harvest. 'Yingqing' peach had one ethylene production peak, and 'Chunxing' strawberry had two -humped curve of ethylene production. The peach and strawberry fruits were treated with exogenous ethylene at concentration of 50 u 1/L. The ethylene production, respiratory rate, rate of superoxide anion radicals production and MDA content were all increased after treatments. The results showed the ethylene has an important role during the fruits ripening.The main physiology-biochemistry changes were determined in the two fruits after treating with trifluoperizine, a caimodulin antagnist, and verapamil, a calcium channel blocker. The results showed that trifluoperizine and verapamil weakened the effects of the exogenous ethylene on the the main physiology-biochemistry. Therefore, calcium messenger took part in the ethylene signal transduction.The microsome membrane Ca2+-ATPase activities increased firstly, then decreased during the two fruits' senescence after harvest. Moreover, the cold temperature (4) treatment delayed the appearance of the microsome membrane Ca2+-ATPase activities peak, and inhibited the decreasing ratio of the microsome membrane Ca2+-ATPase activities. The treatments that could induce the fruits senescence (C2H4, C2H4+TFP, C2H4+Ver) increased the microsome membrane Ca2+-ATPase activities firstly, then promoted the decreasing ratio of the microsome membrane Ca2+-ATPase activities. The results showed that the microsome membrane Ca2+-ATPase activities were closely correlated with the 'Yingqing' peach and 'Chunxing' strawberry fruits senescence.