| 1.Topical preparations for the treatment of mild-to-moderate acne vulgaris:network meta-analysis.Objective: In this study,network meta-analysis was conducted to identify the most effective and best tolerated topical drug treatment for acne.Methods: Relevant literatures published from January 1,2000 to November 30,2022,including the Cochrane,Pubmed,Embase database,CNKI and Wang Fa data.According to the inclusion and exclusion criteria,the literature was screened and extracted data.Results: 1.Total of 28994 mild to moderate acne patients were included in 34 studys,of which 31 literatures recorded the significant efficiency,29 literatures reported the changes in the number of skin lesions,and 32 literatures recorded adverse reactions after treatment;2.Clinical significant efficiency: Clindamycin + BPO+ adapalene gel had the highest clinical efficacy,and the clinical efficacy of the combined application of topical drugs was significantly higher than single.In addition,the efficacy of retinoic acid cream was better than adapalene.Clindamycin gel combined with BPO was also clinically more effective than adapalene;3.Changes in number of lesions: The results were similar to clinical efficiency.The combination of clindamycin+ BPO + adapalene gel had the largest reduction,and the combination of topical drugs was significantly higher than alone.In addition,the combination of Clindamycin +BPO had a higher reduction than adapalene;4.Adverse events: side effects of clindamycin gel is minimal,followed by clindamycin +BPO.Conclusion: The combination of clindamycin gel and benzoyl peroxide gel seems to be the best choice in clinical.Topical drugs containing antibiotics,either monotherapy or combination therapy,are no more effective than non-antibiotics in treating mild-tomoderate acne;The combination of adapalene plus BPO may be more effective than either,but may cause more adverse reactions;The adverse effects of adapalene alone were greater than BPO+clindamycin or alone.2.Lactoferrin regulates sebogenesis and inflammation in vivo and in vitro of acne Objective: Using SZ95 sebaceous gland cells to investigate the anti-inflammation and anti-lipid secretion mechanism of LF,and were verified using a mouse acne model.Methods: 1.Protein and m RNA levels of IL-6,IL-8,TNF-α,TLR2,NF-κB and My D88 were detected by ELISA,RT-PCR and Western-blot after costimulation with different doses of lactoferrin by induction of peptidoglycan in SZ95;2.Insulin was co-cultured with SZ95 sebaceous cells,and then co-stimulated with different doses of LF,and the changes of intracellular lipids were detected by oil red O and Nile red staining,respectively.The contents of SREBP-1,FAS,SCD-1 and ADS-2 in each group were detected by RT-PCR;3.The use of C.acnes and human sebum to constructed of acne in mice model,HE dyeing to observe the modeling,and then were treated with different doses of lactoferrin,following oil red O and Nile red staining to observe sebaceous glands lipid changes in the cell;4.The detection of TUNEL and PCNA expression in acne model change;Western blotting to detect the expression levels of FADS2 and CXCL15 in sebaceous glands.Results: 1.The levels of IL-6,IL-8 and TNF-α m RNA and protein,and TLR2 and its downstream genes NF-κB and My D88 increased in SZ95 cells induced by peptidoglycan,but significantly decreased after LF co-culture.The differences were statistically significant(P < 0.01);2.Cell experiment: LF significantly decreased insulin induced SZ95 sebaceous glands lipid content in cells,oil red O and Nile red staining detection of lipid in cells decreased.The expression levels of FAS,SCD,SREBP-1,FADS-2 and IL-8 were decreased,the difference was statistically significant(P < 0.05);3.Animal experiment: LF treatment acne animal model,the number of sebaceous gland cells decreased significantly,and sebaceous glands cell apoptosis PCNA expression cut and increase,and the organization of FADS2 and IL-8 protein content decreased,the level of the difference was statistically significant(P < 0.05).Conclusions: 1.LF inhibits the expression of acne related inflammatory factors in peptidoglycan-induced SZ95,possibly by inhibiting the TLR2 signaling pathway in the innate immune response,which may be one of the mechanisms of the treatment of acne by LF;2.LF has a significant inhibitory effect on insulin-induced lipid content and related lipid gene expression in SZ95 sebaceous gland cells;3.The animal models to further verify the LF restrain acne lipid secretion and related lipid gene expression. |
